Fig. 2 PRC1 activity is required for leukemic cell growth independently of p16 and p19 expression. PRC1 activity is required for leukemic cell growth independently.

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Fig. 2 PRC1 activity is required for leukemic cell growth independently of p16 and p19 expression. PRC1 activity is required for leukemic cell growth independently of p16 and p19 expression. (A) Relative expression levels of p16 and p19 determined by qRT-PCR analyses in the Cdkn2a-proficient and Cdkn2a-deficient Lin− cells treated for 72 hours with 4-OHT or EtOH (treatment control). Gene expression is normalized to Rpo levels. (B) Western blot analyses with the indicated antibodies on proteins extracted from R26CreERT2Cdkn2a−/−Ring1a−/−Ring1bf/f Lin− cells treated with 4-OHT for 72 hours, demonstrating the loss of Ring1b expression, lack of H2AK119Ub deposition, and absence of p16 expression. EtOH was used as treatment control. Vinculin is presented as loading control. (C) Growth curves of R26CreERT2Cdkn2a−/−Ring1a−/−Ring1bf/f Lin− cells purified from bone marrow and transformed by transduction with lentiviruses expressing the indicated oncogenic proteins. Full PRC1 inactivation in the absence of p16 and p19 transcriptional activation (Cdkn2a−/−) was induced by 4-OHT treatment. EtOH was used as treatment control. Nontransduced cells were used as nontransformed control. (D) May-Grünwald-Giemsa staining of the cells obtained from the experiment presented in (C) at 72 hours after 4-OHT treatment. (E) Methylcellulose colony assays starting from 5000 plated cells, the same presented in (C) at the first and third passages, displayed the transformed phenotype acquired upon expression of the different oncogenes, highlighting a dependency on PRC1 activity independent of Cdkn2a expression. Quantifications of the number of colonies per plate are presented in the right panels. (F) Relative expression levels of Ring1b determined by qRT-PCR analyses in the Lin− cells shown in (C) and (D), showing efficient loss of Ring1b expression upon 72 hours of 4-OHT treatment. Gene expression is normalized to Rpo levels. (G) Relative expression levels of Hoxa9 determined by qRT-PCR analyses in the R26CreERT2Cdkn2a−/−Ring1a−/−Ring1bf/f Lin− cells expressing the indicated oncogenic proteins and treated for 72 hours with 4-OHT or EtOH (treatment control). Gene expression is normalized to Rpo levels and used as a nontransformed control on the nontransduced cells. Alessandra Rossi et al. Sci Adv 2016;2:e1600972 Copyright © 2016, The Authors