Volume 57, Issue 6, Pages 2618-2625 (June 2000) Renal allograft protection with losartan in Fisher→Lewis rats: Hemodynamics, macrophages, and cytokines Farzad Ziai, Hiroaki Nagano, Mamoru Kusaka, Ana J. Coito, Julia L. Troy, Kari C. Nadeau, Helmut G. Rennke, Nicholas L. Tilney, Barry M. Brenner, Harald S. Mackenzie Kidney International Volume 57, Issue 6, Pages 2618-2625 (June 2000) DOI: 10.1046/j.1523-1755.2000.00122.x Copyright © 2000 International Society of Nephrology Terms and Conditions
Figure 1 Proteinuria. Symbols are: () losartan group; (▪) control group. Progressive rises in urinary protein excretion rates seen in the control rats are abolished in rats receiving losartan. *P < 0.05 by ANOVA and Scheffé's test. Kidney International 2000 57, 2618-2625DOI: (10.1046/j.1523-1755.2000.00122.x) Copyright © 2000 International Society of Nephrology Terms and Conditions
Figure 1 Proteinuria. Symbols are: () losartan group; (▪) control group. Progressive rises in urinary protein excretion rates seen in the control rats are abolished in rats receiving losartan. *P < 0.05 by ANOVA and Scheffé's test. Kidney International 2000 57, 2618-2625DOI: (10.1046/j.1523-1755.2000.00122.x) Copyright © 2000 International Society of Nephrology Terms and Conditions
Figure 2 Glomerulosclerosis. The percentage of glomeruli involved with focal and segmental glomerulosclerosis (FSGS) is depicted in a box and whiskers plot. The horizontal margins of the box represent the 75th percentile, and the error bars represent the 90th percentile. The circles denote the range. FSGS in vehicle-treated rats (Control) was characterized by a high variance, whereas the losartan rats showed little variance in sclerosis with four rats scoring zero. Because of the unequal variances between the groups, nonparametric testing was used for statistical comparison (Wilcoxon test, *P < 0.05). Kidney International 2000 57, 2618-2625DOI: (10.1046/j.1523-1755.2000.00122.x) Copyright © 2000 International Society of Nephrology Terms and Conditions
Figure 3 Allograft renal cortical gene expression by semiquantitative RT-PCR. Levels of expression of RANTES, interleukin (IL)-1, IL-6, transforming growth factor-β (TGF-β), inducible nitric oxide synthase (iNOS), endothelin-1 (ET-1), and monocyte chemoattractant protein-1 (MCP-1) were all reduced significantly in losartan rats. Expression of tumor necrosis factor-α (TNF-α) and the classic T-cell products IL-2, interferon-γ (IFN-γ), by contrast, were unaffected in losartan rats. Symbols are: () losartan; (▪) control. *P < 0.05 by Mann–Whitney test. Levels of expression of these molecules under normal conditions in rat renal cortex are all < 0.5. Kidney International 2000 57, 2618-2625DOI: (10.1046/j.1523-1755.2000.00122.x) Copyright © 2000 International Society of Nephrology Terms and Conditions
Figure 3 Allograft renal cortical gene expression by semiquantitative RT-PCR. Levels of expression of RANTES, interleukin (IL)-1, IL-6, transforming growth factor-β (TGF-β), inducible nitric oxide synthase (iNOS), endothelin-1 (ET-1), and monocyte chemoattractant protein-1 (MCP-1) were all reduced significantly in losartan rats. Expression of tumor necrosis factor-α (TNF-α) and the classic T-cell products IL-2, interferon-γ (IFN-γ), by contrast, were unaffected in losartan rats. Symbols are: () losartan; (▪) control. *P < 0.05 by Mann–Whitney test. Levels of expression of these molecules under normal conditions in rat renal cortex are all < 0.5. Kidney International 2000 57, 2618-2625DOI: (10.1046/j.1523-1755.2000.00122.x) Copyright © 2000 International Society of Nephrology Terms and Conditions