Remodeling and maturation of the mouse retinal vasculature.

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Invest. Ophthalmol. Vis. Sci ;57(8): doi: /iovs Figure Legend:

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Remodeling and maturation of the mouse retinal vasculature. Remodeling and maturation of the mouse retinal vasculature. (A) Confocal images showing proliferation (4 hours of EdU incorporation, red) together with Isolectin B4 staining (green) in the retina at the indicated stages. The bottom row shows EdU signals only. Note that proliferation is absent in arteries (arrows) already at P6, but persists in veins (arrowheads) until P14. (B) Quantitative analysis of relative endothelial proliferation at the indicated stages measured by EdU incorporation into retinal arteries, perivenous capillaries and veins as defined by Isolectin B4 staining and morphological criteria. Error bars indicate s.e.m. (C) Whole-mounted P10 and P28 retinas labeled with anti-VE-cadherin antibody (red) and Isolectin B4 (IsoB4, green). The center and bottom rows show higher magnifications of boxed areas 1 and 2, respectively, in the top row. Note the extensive changes in the caliber and endothelial cell (EC) number of vessels (area 1). In contrast to the many ECs found within P10 capillaries, remodeled capillaries in the P28 retina (center row) contained tubules formed by single, hollowed ECs (a, arrowheads mark junctions at both ends), single ECs with autocellular junctions (b), or two ECs with two adherens junctions (c). ECs in veins (bottom row) elongate and align along the flow direction between P10 and P28 (red arrows). Manuel Ehling et al. Development 2013;140:3051-3061 © 2013. Published by The Company of Biologists Ltd