The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

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Volume 5, Issue 2, Pages (October 2013)

Topisirovic et al., (2003) EMBO J,22:

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Presentation transcript:

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. A GFP-fusion of a dominant negative lamin B1 mutant (GFP-Delta 2+) was transiently transfected into HeLa cells. The distribution of lamin A (row A), lamin C (row B), lamin B1 (row C) and lamin B2 (row D) in transfected cells was compared with untransfected cells by antibody staining with JoL2, rabbit anti-lamin C, goat anti-lamin B1 and LN43, respectively. The distribution of DNA was detected with DAPI. Each panel displays either individual black and white images or three-colour merged images in which DAPI is shown in blue, GFP-Delta 2+ in green and antibody staining in red. Bar, 10 μ m. O. Anthony Vaughan et al. J Cell Sci 2001;114:2577-2590 © The Company of Biologists Limited 2001