Fig. 2. Mapping of the interaction domain on coilin for association with the dyskerin complex. Mapping of the interaction domain on coilin for association.

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Fig. 2. Mapping of the interaction domain on coilin for association with the dyskerin complex. Mapping of the interaction domain on coilin for association with the dyskerin complex. (A) Schematic representation of coilin showing the locations of the self-association domain (SA Domain), RNA binding domain (RBD), RG-Box, and TUDOR-like domain (TUDOR) of the various GFP-tagged coilin constructs. (B) Cells were transfected with empty GFP vector or GFP-tagged coilin constructs, followed by IP with antibodies to GFP. Probing antibodies are indicated. For the GFP signals, the same membrane probed with anti-dyskerin was also probed with polyclonal anti-GFP antibodies. The bands for each GFP-tagged protein were then grouped together (lower panel). The experiment was repeated four times, and a representative result is shown. For all IPs, inputs represent 1.5% of cell lysate used in the IP reaction. Aaron R. Poole, and Michael D. Hebert Biology Open 2016;bio.018804 © 2016. Published by The Company of Biologists Ltd