TFEB controls actin cytoskeleton reorganization in response to microbial sensing. TFEB controls actin cytoskeleton reorganization in response to microbial.

Slides:

Advertisements

Similar presentations

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Advertisements

Self-folding triangular devices at two scales.

Localized synthesis of PIP2 by PIP5K is mainly responsible for the PIP2 enrichment outside the BCR microclusters. Localized synthesis of PIP2 by PIP5K.

Specific depletion of TFH and LCMV-specific CD4 T cells.

CD8α+ DC-deficient mice are highly susceptible to Lm infection in the absence of CD169+ macrophages. CD8α+ DC-deficient mice are highly susceptible to.

CD169+ macrophages play a critical role in mediating innate immune cell reorganization. CD169+ macrophages play a critical role in mediating innate immune.

Nuclear calcium signaling drives nuclear actin polymerization in T cells. Nuclear calcium signaling drives nuclear actin polymerization in T cells. (A)

Identification of individual genes that predict vaccination response in young individuals. Identification of individual genes that predict vaccination.

Nuclear Arp3 mediates formation of TCR-induced nuclear actin filaments

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

TCR signaling induces nuclear actin polymerization.

Inhibition of macropinocytosis activates the TFEB-TRPML1 axis.

Altered distribution of γδT IELs in GPR55-deficient mice.

Cytosolic entry of Lm required for CD8α+ DC recruitment.

VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination

Tukey boxplots overlaid on data points from objective and subjective measures, displaying results from study 1. Tukey boxplots overlaid on data points.

Characterization of the light-responsive transdermal MNs.

Low accessibility to Ag for CTLs leads to low proliferation of effectors. Low accessibility to Ag for CTLs leads to low proliferation of effectors. Intravital.

NCMs regulate T cell survival in TLOs via PD-L1.

In vivo tracking of PD-L1+NCMs in the microcirculation.

Fig. 4 3D reconfiguration of liquid metals for electronics.

Aggregation kinetics of Aβ16–22 are unaffected by the presence of Aβ40

Crystal structures of RSV G-antibody complexes.

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Effect of slenderness on optimal shapes.

CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

2D motility characterization and external magnetic steering of RBC microswimmers. 2D motility characterization and external magnetic steering of RBC microswimmers.

Fig. 4 Super-resolution live imaging of calcium containing vesicle transports via lysosomes. Super-resolution live imaging of calcium containing vesicle.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 2 Ferroelectric domains resolved in WTe2 single crystals.

Interactive morphogenesis in Ch-CNC droplets laden with magnetic NPs

Full-frame single image recording the violation of a Bell inequality

Fig. 1 Cryo-EM structure of yeast Elp123 showing its active site.

Fig. 1 Bioinspired design of AAD for promoting wound contraction.

The two modalities for the multitask condition.

CsEN components reveal precisely timed cellular programs that characterize the dynamic changes of the peripheral immune system over the course of pregnancy.

Characterization of the MCs.

Tfr cells respond better to immunization with self-antigens than with foreign antigens. Tfr cells respond better to immunization with self-antigens than.

TRPML1 is required for fast DC migration, chemotaxis, and arrival at the LNs. TRPML1 is required for fast DC migration, chemotaxis, and arrival at the.

Electronic structure of the oligomer (n = 8) at the UB3LYP/6-31G

Fig. 1 Operation mechanism and scanning electron microscopy images of MTC device. Operation mechanism and scanning electron microscopy images of MTC device.

Galloping-like gait with the design of a two-legged robot.

AEGIS autonomous targeting process.

Fig. 3 Hydrostatic pressure induces intracellular calcium increase via TRPM7 activation. Hydrostatic pressure induces intracellular calcium increase via.

Fig. 3 ET dynamics on the control and treatment watersheds during the pretreatment and treatment periods. ET dynamics on the control and treatment watersheds.

Human Tfr cells do not express CD25.

Deformability and stability of RBC microswimmers squeezing through confined spaces smaller than their size. Deformability and stability of RBC microswimmers.

Fig. 1 Structure and absorption spectra of the P

Calcium release through TRPML1 controls myosin IIA retrograde flow at the rear of LPS-DCs. Calcium release through TRPML1 controls myosin IIA retrograde.

Fig. 4 BS-SEM images, ternary diagrams, and phase maps for the text and reverse sides of the TS. BS-SEM images, ternary diagrams, and phase maps for the.

Multiplexed four- and eight-channel devices for rapid processing

Long-range structural order control of SS-annealed cylinder patterns

Fig. 5 Simulation of the mechanical properties of the 3DGraphene foam in a wide temperature range down to the cryogenic region. Simulation of the mechanical.

Fig. 2 Particles detected in snow samples collected at different locations from Europe to the Arctic. Particles detected in snow samples collected at different.

Fig. 1 TFP distribution and deviations.

Fig. 4 Single-particle contact angle measurements.

Fig. 3 Supraballs and films assembled from binary 219/217nm SPs/SMPs.

Fig. 2 Supraballs and films from binary SPs.

Fig. 1 Schematic structure of a fluorescently labeled eGLP1-conjugated MALAT1 ASO and internalization of fluorescent eGLP1 and eGLP1-MALAT1-ASO. Schematic.

Fig. 1 Design principle and SEM characterization of super-origami DNA nanostructures with n-tuples. Design principle and SEM characterization of super-origami.

Meningeal γδ T cells are biased toward IL-17 production.

Fetal-derived γδ T cells infiltrate the meninges from birth.

Fig. 3 Neuroblast-induced morphological dynamics of reactive astrocytes involve the inhibition of Cdc42-regulated actin polymerization. Neuroblast-induced.

Fig. 1 Differential anterograde transport of CCR5 and TNF.

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Fig. 1 Sacrificial writing into functional tissue (SWIFT).

Fig. 2 Imaging blood vessel before and after closure.

Comparison of children’s behavior between the three conditions.

Presentation transcript:

TFEB controls actin cytoskeleton reorganization in response to microbial sensing. TFEB controls actin cytoskeleton reorganization in response to microbial sensing. (A) LifeAct-GFP immature DCs and LPS-DCs migrating in 8 μm–by–5 μm fibronectin-coated microchannels. The middle (M) and cortical (C) planes were imaged. The inset (rectangle with a dashed boundary) highlights the proximity of the lysosomes (in red, labeled with WGA AF-647) to the F-actin patch observed in LPS-DCs. The red arrowhead indicates an actin cable that originates in the actin patch and extends toward the cell rear (spinning disk microscope, ×100 magnification; scale bars, 5 μm). (B) Sequential images of LifeAct-GFP siCtrl or TFEB-silenced (siTFEB) LPS-DCs (8 μm–by–5 μm fibronectin-coated channels; epifluorescence microscope, ×20 magnification; one image every 2 min; scale bars, 10 μm). (C) Mean LifeAct-GFP distribution in siCtrl or siTFEB DCs (from top to bottom, n = 40, 38, 29, and 35 cells; representative results from one of two experiments are shown). (D) Dynamic analysis of the fraction of time spent by the cells depicted in (C) with LifeAct-GFP at their front. (E) The mean F-actin front-back ratio. (D) Data are quoted as means, and error bars correspond to SEM. (E) Data are represented as boxes and whiskers; bars include 90% of the points, the line represents the median, and the box contains 75% of the data. Mann-Whitney test was applied. Marine Bretou et al. Sci. Immunol. 2017;2:eaak9573 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works