Subcellular distribution of TACE-eGFP changes with the coexpression of active ERK2. Subcellular distribution of TACE-eGFP changes with the coexpression.

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Subcellular distribution of TACE-eGFP changes with the coexpression of active ERK2. Subcellular distribution of TACE-eGFP changes with the coexpression of active ERK2. (A) HeLa cells were transfected with either 0.3 μg pWT.TACE-GFP or pT735A.TACE-eGFP, with or without 0.1 μg of pCA.MEK-1.HA and/or pWT.ERK2-HA. DNA amounts were kept equivalent using pcDNA3.1+/Zeo. Cells were fixed in methanol and stained for constitutively active MEK-1 (α-MEK-1), WT.ERK-HA (α-HA), or active phosphor-ERK2 (α-pERK2 in B), before being visualised using laser-scanning confocal microscopy. TACE-eGFP, CA.MEK-1 and/or ERK2 are green and red respectively, whereas the nuclei are blue. The arrowheads highlight a change in the subcellular location of TACE-eGFP protein. Bar, 10 μm. Surinder M. Soond et al. J Cell Sci 2005;118:2371-2380 © The Company of Biologists Limited 2005