The mucosal environment regulates CD160 expression on IELs The mucosal environment regulates CD160 expression on IELs. Naive CD8+ T cells were transferred into RAG−/− recipients and isolated 10 d later from mLN (blue), spleen (black), and colonic IELs (red) and analyzed for (A) CD160 expression levels and (B) percentages of cells expressing CD160. The mucosal environment regulates CD160 expression on IELs. Naive CD8+ T cells were transferred into RAG−/− recipients and isolated 10 d later from mLN (blue), spleen (black), and colonic IELs (red) and analyzed for (A) CD160 expression levels and (B) percentages of cells expressing CD160. (C) Percentages of colonic IELs expressing CD160 from age- and sex-matched WT (blue) and P14 TCR transgenic mice (red). Representative plots are shown to the left and summary of CD160-expressing IELs to the right. (D) CD160 expression levels on CD8+ T cells that were unstimulated (gray) or activated with anti-CD3 in the presence of IL-10 (blue), TGF-β (green), IL-10 plus TGF-β (purple), and anti-CD3 alone (black). Representative plots are shown to the left and summary of CD160 mean fluorescence intensity (MFI) to the right. (E) CD160 expression levels and percentages of TCRβ+ and TCRγδ+ colonic IELs expressing CD160 from age- and sex-matched WT (blue) and IL-10−/− mice (red). Representative plots are shown to the left and summary of CD160-expressing TCRβ+ and TCRγδ+ colonic IELs to the right. Data are represented as means ± SEM. The results are representative of at least two independent experiments. Significance was assessed using one-way ANOVA in (B) and (D) and Student t test in (C) and (E). *p < 0.05, ***p < 0.005, ****p < 0.001. Catherine L. Tan et al. ImmunoHorizons 2018;2:238-250 Copyright © 2018 The Authors