Crotonate increases histone crotonylation in mouse kidney. Crotonate increases histone crotonylation in mouse kidney. Mice were treated with crotonate at different doses and for different times. Histone crotonylation was measured by western blotting (anti-Kcr). (A) Dose-response curve at 48 h. Mice received vehicle, or 3, 6 or 12 mmol/kg body weight crotonate by intraperitoneal injection. Mean±s.e.m. of four mice per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (B) Timecourse curve. Mice were killed 24, 48 or 72 h following intraperitoneal injection injection of 12 mmol/kg body weight crotonate or vehicle (control). Mean±s.e.m. of four mice per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). Olga Ruiz-Andres et al. Dis. Model. Mech. 2016;9:633-645 © 2016. Published by The Company of Biologists Ltd