Fig. 1. Exogenous folic acid and Folr1 rescues the function of a Rho-kinase binding mutation in Shroom3. Exogenous folic acid and Folr1 rescues thefunction.

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Volume 27, Issue 9, Pages (May 2017)

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Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis.

Fig. 7. Vinculin recruitment enhances the efficiency of barrier formation.(A) TER measurements after a calcium switch in α-catenin-depleted MDCK cells.

Fig. 2. Outline of the two types of stimulus sequences employed in the analysis.(A) Environment information stimuli; (B) adaptation stimuli. Outline of.

Fig. 8. In vitro effect of CMPs on adult DSS-treated stomach explants.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 2. Proportion of motile objects and track length

Fig. 6. Hts regulates par-1 and camkII mRNA distribution and levels in the muscle.(A–J) Views of muscles 6/7 in abdominal segment 4, probed for either.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

Fig. 10. Ratiometric live imaging of di-4-ANEPPDHQ in growing pollen tubes.(A) Higher and lower membrane order distribution in control and BCD treated.

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

Fig. 2. Mapping of the interaction domain on coilin for association with the dyskerin complex. Mapping of the interaction domain on coilin for association.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Colocalization of the palmitoylation-deficient mutants of claudin-14 with the lysosomal membrane protein LAMP-2. Colocalization of the palmitoylation-deficient.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 2. Transfection and clonal selection of rat pluripotent stem cells to generate stable transgenic lines. Transfection and clonal selection of rat pluripotent.

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. E-cadherin localizes in nano-scale clusters.

PfSec13 does not co-localize with P. falciparum heterochromatin.

Fig. 3. Mutation of Y520 and Y667 result in increased delivery of prestin to the apical surface of MDCK cells.MDCK cells transiently transfected with wt.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 6. Localization of Sma during growth and migration

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 2. Soluble sugar and organic acid levels with different K fertilization during fruit development. Soluble sugar and organic acid levels with different.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Fig. 6. STK35 KO mice show ovary defects.

Fig. 2. Sorting in chimeric embryoid bodies of wildtype with E- or N-cadherin null ES cells.Wildtype, E-cadherin null (9J), and N-cadherin null (Ncad95)

Fig. 6. Apical polarity of primitive endoderm cells on surface of embryoid bodies.Embryoid bodies were analyzed by immunofluorescence microscopy for GATA4.

Reb does not play a significant role in regulating TGF-β signaling

Fig. 6. Meis1 protein is present in CR4. 2-GFP+ and Foxn4+ cells

Fig. 3. Overexpression of wild-type GFP-CPAP, but not GFP-CPAP-377EE (GFP-377EE), induces cilia formation and promotes the growth of cilia.CAD cells (A,B)

Effect of the plastid translation inhibitor Spec on LR development

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Fig. 3. Exogenous folic acid rescues neural epithelial apical constriction and activation of non-muscle myosin upon Rho-kinase inhibition. Exogenous folic.

Analysis of Cdc7p at the end of meiosis.

Folic acid increases apical junctional pMLCK localization in vivo

Fig. 2. Non-homogeneous subcellular distribution of Vangl2 along the anteroposterior axis. Non-homogeneous subcellular distribution of Vangl2 along the.

dcn1-deletion results in attenuated cohesin cleavage at anaphase

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

The expression of two forms of N-cadherin.

Fig. 2. Coupling of actin to cell–cell junctions requires α-catenin and is necessary for the establishment of the barrier.(A) Effect of Cytochalasin D.

Wrch-1 localizes to focal adhesions.

Fig. 2. Expression of Cx43 mutant T154A resulted in non-radial spreading and formation of protrusions in J558µm3 cells spreading in response to BCR signaling.(A)

Fig. 4. CR4.2 may be active in amacrine cells but not in ganglion cells.Chick retinas were electroporated with either the control CAG-GFP construct or.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Fig. 5. Testis defects in STK35 KO mice.

Fig. 3. MO-mediated smc3 knockdown results in reduced regenerate length, segment length and cell proliferation. MO-mediated smc3 knockdown results in reduced.

Fig. 1. p85β localizes at adhesion plaques and associates with FAK

Elevated levels of tRNAiMet promote α5β1-dependent cell migration

Association of NM-HA and NM-GFP with SGs is transient.

RA signaling in early postnatal PFC

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

Presentation transcript:

Fig. 1. Exogenous folic acid and Folr1 rescues the function of a Rho-kinase binding mutation in Shroom3. Exogenous folic acid and Folr1 rescues thefunction of a Rho-kinase binding mutation in Shroom3. (A–F) Apical views of MDCK cells transfected with the indicated expression vector, incubated with or without exogenous folic acid (100 µM) and immunofluorescently labeled with β-catenin (turquoise) and either Shroom3 or Folr1 antibodies (red). Above each panel is a virtual section through the transgenic cell in the x-z plane. Scale bar: 20 µm. (G) The mean apical basal area ratio (ABAR) was calculated from area measurements of apical and basal images of transgenic cells and depicted in the graph. Asterisks indicate data sets with significantly reduced ABAR ratios P<0.01. (H) The shapes depict the approximate shape range of cells with the indicated ABARs in increments of 0.3. The percentage of cells within specific ABAR increments are represented for the indicated experimental groups. Arrows mark the ABAR increment group with the greatest number of cells. Note that the experimental groups have distinct peak ABAR increment ranges indicating a shift in the population. (I) Time-lapse images of a Shroom3R1838C-mCherry tagged transgenic cell before and after the addition of folic acid (100 µm). (J) The apical outline of the transgenic cell in I was traced at each time point and superimposed to show the radial reduction of apical area. (K–L) The change in apical area was plotted with time following folic acid treatment of four representative transgenic cells expressing either N-cadherin-GFP or Shroom3R1838C-mCherry. Jessica B. Martin et al. Biology Open 2019;8:bio041160 © 2019. Published by The Company of Biologists Ltd