Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos at E12.5. Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos.

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Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos at E12.5. Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos at E12.5. (A-C) Foxa2 (FP) and Nkx2.2 (p3) expression in WT, Nkx2.2p3-CRM::Cre;ShhΔflox/Δflox and Nestin::Cre;SmoΔflox/Δflox transgenic lines. (D-F) Olig2 (pMN) and Nkx2.2 (p3) expression at lumbar levels. Note the absence of Olig2 expression (arrowhead in D indicates expression in WT) in ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos (asterisks). (G-I) Chx10 (V2a interneurons) and Nkx2.2 expression. Bracket in G indicates the pMN domain (located between the p3 and p2 domains) in WT embryos that is absent in ShhΔFP/ΔFP or SmoΔNT/ΔNT embryos. (J,K) Expression of Chx10 and β;-gal in Nkx2.2p3-CRM::Cre mice. In WT embryos, Chx10+ cells are generated from the p2 lineage that does not activate expression of Nkx2.2p3-CRM (as indicated by β;-gal expression) (Wang et al., 2011), whereas in ShhΔFP/ΔFP embryos lacking Olig2 expression some Chx10+ cells co-express β;-gal (K, yellow cells, arrowhead). (L) Expression of Olig2 is substantially rescued in Nkx2.2p3-CRM::Cre;ShhΔflox/Δflox;Gli3xt/xt mice that also lack Gli3. Thoracic level is shown. (M-O) Cell counts for each marker for the indicated genotypes. **P=0.07; *P<0.001; +P<0.0001. Student’s two-tailed t-test. Error bars indicate s.e.m. Scale bars: 50 μm. Kwanha Yu et al. Development 2013;140:1594-1604 © 2013. Published by The Company of Biologists Ltd