Fig. 4 Pathological features of c9FTD/ALS correlate with SUPT4H1 and SUPT5H expression in patient brain tissue, and partial knockdown of SUPT4H1 in C9orf72.

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Fig. 4 Pathological features of c9FTD/ALS correlate with SUPT4H1 and SUPT5H expression in patient brain tissue, and partial knockdown of SUPT4H1 in C9orf72 iPSC-derived cortical neurons reduces production of C9orf72 variant 3 mRNA and DPR proteins. Pathological features of c9FTD/ALS correlate with SUPT4H1 and SUPT5H expression in patient brain tissue, and partial knockdown of SUPT4H1 in C9orf72 iPSC-derived cortical neurons reduces production of C9orf72 variant 3 mRNA and DPR proteins. (A and B) SUPT4H1 and SUPT5H mRNA levels in cerebellar tissue from c9FTD/ALS patients were measured by qPCR and found to associate with cerebellar levels of C9orf72 variant 3 mRNA measured by qPCR (A) and with poly(GP) proteins measured by immunoassay (B) using a Spearman’s test of correlation. (C to E) Cortical neurons differentiated from each C9orf72 iPSC line were aged for 8 weeks and then transduced with lentivirus expressing either a noncoding control shRNA or an shRNA against SUPT4H1 mRNA for 6 days. Four iPSC lines derived from three patients with C9orf72 repeat expansions were used. Knockdown of SUPT4H1 mRNA (C) and C9orf72 variant 3 mRNA expression levels (D) were determined by qPCR. Poly(GP) protein levels were examined by immunoassay (E). *P < 0.05, **P < 0.01, ****P < 0.0001 as assessed by two-tailed unpaired t test from three independent neuronal differentiations. Nicholas J. Kramer et al. Science 2016;353:708-712 Copyright © 2016, American Association for the Advancement of Science