Volume 131, Issue 4, Pages 1262-1270 (October 2006) A Tumor Progression Model for Hepatocellular Carcinoma: Bioinformatic Analysis of Genomic Data  Terence C.W. Poon, Nathalie Wong, Paul B.S. Lai, Magnus Rattray, Philip J. Johnson, Joseph J.Y. Sung  Gastroenterology  Volume 131, Issue 4, Pages 1262-1270 (October 2006) DOI: 10.1053/j.gastro.2006.08.014 Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 1 An evolution tree for classifying the 158 HCC cases into 4 classes at 3 different evolution levels was constructed from the significant CGH events by SOTA. The class closest to the root was assigned as SOTA 1, and the 1 in the middle as SOTA 2. There were 2 classes at the bottom taxonomy level, which were grouped as SOTA 3. There were 2 subgroups A and B in the SOTA 3. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 2 Recurrence-free survival analysis of the HCC patients classified by SOTA. Among the 112 HCC patients who received curative tumor resection, the SOTA 3A group had a poorer recurrence-free survival compared to the SOTA 1, SOTA 2, and SOTA 3B groups. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 3 The frequency patterns of minimal overlapping regions of the CGH events in the 3 SOTA groups 1, 2 and 3, that potentially represent early (A), intermediate (B), and late-stage (C) events. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 4 Hierarchical clustering of the CGH events of SOTA 2 (A) and SOTA 3 (B). *The predicted earlier event(s) that demonstrated the highest occurrence frequency in each branch. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 5 Proposed evolutionary changes in the HCC carcinogenetic pathway. For chromosomal imbalances in the intermediate and late tumor stage, the minimal overlapping regions are presented. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions

Figure 6 Overall survival (A) and recurrence-free survival (B) analyses of the HCC patients with and without gain of 3q22-24. Gastroenterology 2006 131, 1262-1270DOI: (10.1053/j.gastro.2006.08.014) Copyright © 2006 American Gastroenterological Association (AGA) Institute Terms and Conditions