The Health Benefits of Isotonix OPC-3® Mark Lange, Ph.D. James Wilmer, Ph.D. Market America, Inc.

Statements have not been evaluated by the Food and Drug Administration Statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Primary Benefits Although there are many potential uses for Isotonix OPC-3®, the most common are: Helps maintain normal, healthy circulation Helps promote cardiovascular health.

Pharmacological Actions Helps maintain normal blood glucose levels Supports normal platelet activity Powerful free radical scavenger Demonstartes anti-inflammatory action Supports healthy endothelial function

Serving Size The serving size for a healthy individual for general health maintenance is given on the label (one capful per day). Consult with your healthcare provider before taking additional servings per day.

Duration of Use Most people see the benefit of Isotonix OPC-3® within 8-12 weeks. Given Isotonix OPC-3’s long history of use by thousands of satisfied customers, no limitation on length of use is indicated.

Quality of Ingredients Isotonix OPC-3® is blended using the finest ingredients available. Each ingredient is affirmed GRAS (generally recognized as safe), free of pesticide residue and in compliance with USP and FDA requirements for purity.

Isotonix OPC-3® Contains Pycnogenol® Pycnogenol is a proprietary extract of the French maritime pine bark. Pycnogenol contains oligomeric proanthocyanidins (OPCs) as well as other bioflavonoids. Only Isotonix products have Pycnogenol in isotonic delivery form.

Proanthocyanidins are found in grape seed extract, pine bark and red wine.

How do OPCs Protect Tissue from Free Radical Attack? They donate electrons from the hydroxyl (–OH) group. Donated electron stabilizes (inactivates) free radicals. Then forms a stable intermediate that will not react with tissue. Vitamin C recycles (regenerates) OPCs’ antioxidant capability.

OPCs Help Protect Tissues and Cells Lipids, proteins and DNA are targets for free radical attack. Free radicals not only directly cause cell injury, they can also act as signals for pro-inflammatory pathways. Helps prevent oxidative stress in the human body.

Human Cells have fat-like and water-like regions; where water-soluble and fat-soluble antioxidants reside Lipid bilayer (cell wall) Fat-like region Cytoplasm Water-like region

OPC-3® and Cardiovascular Health Endothelial cells line the inner wall of blood vessels. Endothelial cell health is an important factor in cardiovascular health. The endothelium also helps control blood pressure through vasorelaxation. Pycnogenol stimulates nitric oxide production which promotes normal vasorelaxation (dilation of blood vessels).

Accelerated antioxidant bioavailability of OPC-3® bioflavonoids administered as isotonic solution Cesarone et al. Phytotherapy Research 23, 775-777(2009) 10 subjects (5 male, 5 female), 24-45 years, healthy, non-smokers with elevated plasma oxidative stress Subjects took: OPC-3 first in isotonic form, then 1 week later OPC-3 in tablet form (with equivalent water) Plasma antioxidant effect was monitored over a 4 hour period. In this study, the antioxidant capacity of OPC-3 in isotonix form was compared against OPC-3 in tablet form.

Isotonic OPC-3® versus OPC-3® in tablet form. Reduced plasma oxidative stress (increase in plasma radical reduction rate to baseline) within 10 minutes. Plasma free radical values always remained lower throughout the 4 hours. Biphasic absorption noted: first phase- rapid absorption and antioxidant effect from monomeric and dimeric species. second phase- may involve absorption of larger molecules, maximizing at 70 mins.

Isotonix OPC-3 reduces free radicals faster. OPC-3 in tablet form never quite equals antioxidant power of Isotonix OPC-3.

In this publication, the authors conclude: “In conclusion the findings demonstrate that the flavonoid mixture provided in isotonic OPC-3® is significantly more bioavailable in humans, in terms of antioxidant activity than an equivalent mixture in tablet form.”

Summary Isotonix OPC-3® provides isotonic delivery of Pycnogenol, along with other sources of active bioflavonoids. Isotonix OPC-3® promotes cardiovascular health and normal flood vessel function. Isotonix OPC-3® is a safe, all-natural supplement known for a wide range of health benefits.