The developmental stages investigated in this study.

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Volume 3, Issue 2, Pages (August 2002)

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The developmental stages investigated in this study. The developmental stages investigated in this study. Fourth-instar larvae that developed a new cuticle 30 h before ecdysis (pharate fifth-instar larvae), last larval stage (fifth instar) just after ecdysis (day 0) and during feeding on days 1–4, wandering on days 5–6 (larvae that stop feeding and crawl to find a place for pupation) and post-wandering on days 7–9 (pharate pupae), freshly ecdysed pupae and pupae on days 1–5, developing adults on days 10 and 15, pharate adults 1 day prior to emergence and freshly ecdysed adults. The relative ecdysteroid titres shown in the trace were taken from Žitňan et al. (Žitňan et al., 1999), for pharate fifth-instar larvae, from the present study for fifth-instar larvae and pharate pupae and from Bollenbacher et al. (Bollenbacher et al., 1981) for pupal–adult development. Inka Žitňanová et al. J Exp Biol 2001;204:3483-3495 © The Company of Biologists Limited 2001