Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

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Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr with no stimulant (0), with NGF (N, 100 ng/ml), with rapamycin in the last 24 hr (R, 0.1 µM) and with the two together (NR). Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr with no stimulant (0), with NGF (N, 100 ng/ml), with rapamycin in the last 24 hr (R, 0.1 µM) and with the two together (NR). The quantization of the data is on the right panels. The levels of the S6 and Akt proteins were not changed by the treatments. The numbers flanking the gels are the MDa of the immunolabeled proteins. Statistical analysis and significance of the differences is given as specified in the legend for Fig. 1. Sara Negrini et al. Biology Open 2013;bio.20135116 © 2013. Published by The Company of Biologists Ltd