BRI1 signaling at the dividing cells restores overall root growth

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BRI1 signaling at the dividing cells restores overall root growth BRI1 signaling at the dividing cells restores overall root growth AEight‐day‐old seedlings of WT, bri1‐116, and pRPS5a:BRI1:YFP;bri1‐116. Scale bar 1 cm.BRoot length measurements of 10‐day‐old bri1‐116, bes1‐D, and pRP5SA:BRI:YFP;bri1‐116 seedlings compared to the wild type. Symbols represent the mean of more than 20 plants, from three independent experiments. Error bars show standard deviation. Straight lines represent the linear regression applied to each curve starting with day 4 postgermination. In this way, the root growth rate can be extracted for each phenotype (see Table EV7).CJuxtaposed (red lines) confocal images of 8‐day‐old WT, bri1‐116, and pRP5SA:BRI:YFP;bri1‐116 roots stained with PI. Green lines label the end of the transition zone, and the yellow lines label the first root hair (end of elongation zone). The inset shows pRP5SA expression domain of an 8‐day‐old pRP5SA:BRI:YFP;bri1‐116 seedling. Green line labels the end of the meristematic zone, which coincides with the end of pRP5SA expression domain. Scale bars correspond to 100 μm.D–IMain relationships as in Fig 2C–E between experimental values of the phenotypic traits used to assess the mechanism of differentiation in bri1‐116 mutant (D, F, H) and pRP5SA:BRI:YFP;bri1‐116 line (E, G, I) for the epidermis (left large plot) and cortex (right inset plot). Each circle denotes data extracted from a single root (n = 126 (epidermis) and n = 25 (cortex) for bri1‐116 and n = 90 (epidermis) and n = 17 (cortex) for pRP5SA:BRI:YFP;bri1‐116, see Table EV1, pooled from 6‐, 8‐, and 10‐day‐old seedlings for epidermis and from day 6 for cortex [data in Table EV5)]. In (H, I), the slope of the linear regression for epidermal data is 2.13 ± 0.04 for bri1‐116 and 2.53 ± 0.04 for pRP5SA:BRI:YFP;bri1‐116. Pearson correlation coefficient r is indicated and P‐value (P, using standard Pearson correlation test). In (F), bri1‐116 epidermal data do not conform to normal distributions (Spearman correlation test results in (epidermis) r = −0.29, P = 0.00104 and (cortex) r = −0.54, P = 0.00546 for bri1‐116, and in (epidermis) r = −0.24, P = 0.020 and (cortex) r = −0.38, P = 0.136 for pRP5SA:BRI:YFP;bri1‐116). Irina Pavelescu et al. Mol Syst Biol 2018;14:e7687 © as stated in the article, figure or figure legend