Indirect fluorescence assay of N. gruberi cells.

Slides:

Advertisements

Similar presentations

Immuno-gold localisation of COPI in N. gruberi cells.

Advertisements

Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,

Endogenous SRP19 is localized to the nucleus and the nucleolus.

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

Alveolar lymphangiogenesis is a feature of idiopathic pulmonary fibrosis (IPF). a) Tissue sections reacted with anti-D2-40 (brown) and anti-CD34 (red)

Candidates with a PVM localization.

Cholera Toxin FhHDM-1 Merged

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.

FAM134B-2 colocalizes with SERCA2 and SEC62.

Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial cells using live-imaging. Evaluation of LC3 and ATG13 dynamics in AS-stimulated microglial.

Occurrence of prominin-1-containing particles in the ventricular fluid of the embryonic mouse brain. Occurrence of prominin-1-containing particles in the.

Fig. 1. Overview of the nervous system of the adult S. roscoffensis.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Localisation of epithelial markers in mMEC cultures.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Function and localization of nuclear UPS components.

The distribution of lamin C and emerin in lymphoblastoid cell lines.

Fig. 2. abu/pqn genes are expressed in the pharyngeal cuticle

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Fig. 1. Mitochondrial internalization in cardiomyocytes.

Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. Neutralization and knockdown of TGFβ2 results in fewer nephrons ex vivo. (A,B)

Fig. 2. Transfection and clonal selection of rat pluripotent stem cells to generate stable transgenic lines. Transfection and clonal selection of rat pluripotent.

Fig. 5. Recovery steps of mitotic acentriolar cells after cold MT depolymerisation.(A,B) Time-lapse sequences of mitotic spindle re-formation at 18°C.

PfSec13 does not co-localize with P. falciparum heterochromatin.

The initial targeting of Sec61b mRNA to the ER is partially dependent on ribosomes and translation. The initial targeting of Sec61b mRNA to the ER is partially.

RNAi-induced loss of Ce-emerin protein persists into adulthood.

Kinetics of induced and non-induced AR, and its relationship to cell death and motility. Kinetics of induced and non-induced AR, and its relationship to.

Fig. 6. LR phenotype of plastid translation-defective mutants with/without Spec. LR phenotype of plastid translation-defective mutants with/without Spec.

The coding potential of GFP-Sec61b is not required for its localization to the ER. (A) COS7 cells were transfected with plasmid encoding various GFP-tagged.

Fig. 6. Comparison of Plk4 with Sas-6 localization

Α-Catenin DKO cardiomyocytes exhibit increase proliferation on stiff ECM. (A) Representative images of neonatal cardiomyocytes cultured on different PA-FN.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.

H1. X depletion by RNA interference and colocalization of H1

Acridine orange (red)- and DAPI (yellow)-stained nematocysts in tentacles of Hydra. Acridine orange (red)- and DAPI (yellow)-stained nematocysts in tentacles.

Axial organisation of gastruloids.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Colocalisation and influence on migration.

Characterization of anti-N-mPres.

Nerve conduction slowing and DRG sensory neuron and nuclear atrophy in 16-week-old diabetic mice. Nerve conduction slowing and DRG sensory neuron and nuclear.

Z-stack compressions showing triple labelling of different cell types in the gills of bowfin with antibodies for serotonin (5-HT, green) and HNK-1 (magenta),

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

The actin organization and N-cadherin dynamics in migrating cells.

SLK/LOK-phosphorylated and activated ezrin prevents MISP localization at the cell cortex. SLK/LOK-phosphorylated and activated ezrin prevents MISP localization.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Fig. 2. iPSCs produce functional osteoblasts.

Immunofluorescence analysis of GpGCS1 localization in strains K41 (mating-type plus) and K34 (mating-type minus). Immunofluorescence analysis of GpGCS1.

Specific upregulation of MKL1_S during the initial phase of TGF-β-induced myofibroblast differentiation. Specific upregulation of MKL1_S during the initial.

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Quantitative evaluation of isolated Calliphora rhabdoms labelled for Rh3, Rh5 and Rh6. Quantitative evaluation of isolated Calliphora rhabdoms labelled.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Depletion of EB1 with small interfering RNA reduces MT minus-end anchoring at the centrosome. Depletion of EB1 with small interfering RNA reduces MT minus-end.

The BRCA1 aggregates exclude large nuclear structures.

Depletion of COPI protein inhibits cis to trans cisternal maturation.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Sensory neurons grow and downregulate UCHL1 expression levels during postnatal maturation. Sensory neurons grow and downregulate UCHL1 expression levels.

Conservation of key sequence features and endocytic localisation in TbCALM. Conservation of key sequence features and endocytic localisation in TbCALM.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

BMOL3 LPCs can differentiate towards the hepatocyte lineage.

Telomere cohesion is established in S phase in cohesin-ring-depleted cells. Telomere cohesion is established in S phase in cohesin-ring-depleted cells.

Analysis of centromere and kinetochore function after depletion of DmSMC4. Analysis of centromere and kinetochore function after depletion of DmSMC4. (A)

Subnuclear localization of HA-tagged EBNA1 protein.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Generation of an inducible form of FOXN1.

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Presentation transcript:

Indirect fluorescence assay of N. gruberi cells. Indirect fluorescence assay of N. gruberi cells. (A) Cellular localisation of COPI in N. gruberi cells. Chicken anti-N.gruberi COPB antiserum (1:250; green) shows a discrete localisation in the cells, while DAPI stains the Naegleria nucleus and mitochondrial DNA. Differential interference contrast (DIC) images show the cells used for immunofluorescence. (B) Cellular localisation of Sec31 in N. gruberi cells. Rat anti-N.gruberi Sec31 antiserum (1:250; red) shows a discrete localisation in the cells, while DAPI stains the Naegleria nucleus and mitochondrial DNA. DIC images show the cells used for immunofluorescence. (C) Cellular localisation of COPI and Sec31 in N. gruberi cells. Chicken anti-N.gruberi COPB antiserum (green) shows a discrete localisation in the cells that is not co-localised with the rat anti-N.gruberi Sec31 antiserum (red), while DAPI stains the Naegleria nucleus and mitochondrial DNA. DIC images show the the cells used for immunofluorescence. The experiment was performed three times in six replicates. Scale bars: 10 μm. Emily K. Herman et al. J Cell Sci 2018;131:jcs213306 © 2018. Published by The Company of Biologists Ltd