Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells

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Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells.(A) Identification of Cbx7−/− clones by qRT-PCR (upper panel) and Western blot (lower panel) analyses. Increased adipocyte differentiation in Cbx7-KO ES cells.(A) Identification of Cbx7−/− clones by qRT-PCR (upper panel) and Western blot (lower panel) analyses. (B) Oil Red O staining of lipid droplets in Cbx7+/+, Cbx7+/− and Cbx7−/− ES-derived adipocytes. Pictures were taken on a bright field microscope (Zeiss) at a magnification of 80×. (C) Quantification of Oil Red O incorporation by measuring the absorbtion at 500 nm of Oil Red O extracted from cells shown in B. (D) qRT-PCR analyzing the expression of Cd34, Cebpb, Pparg, Ap2 and leptin genes in ES cells before and after 9 and 15 days of adipocyte differentiation. Floriana Forzati et al. Biology Open 2014;3:871-879 © 2014. Published by The Company of Biologists Ltd