Wnt6 maintains anterior escort cells to promote GSC survival.

Slides:

Advertisements

Similar presentations

Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn. Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn.

Advertisements

Volume 1, Issue 4, Pages (October 2007)

Volume 9, Issue 1, Pages (July 2017)

Volume 15, Issue 4, Pages (February 2005)

Volume 16, Issue 23, Pages (December 2006)

Volume 9, Issue 5, Pages (November 2005)

Lacy J. Barton, Belinda S. Pinto, Lori L. Wallrath, Pamela K. Geyer

Volume 27, Issue 6, Pages (March 2017)

Drosophila piwi Mutants Exhibit Germline Stem Cell Tumors that Are Sustained by Elevated Dpp Signaling Zhigang Jin, Alex S. Flynt, Eric C. Lai Current.

Dcr-1 Maintains Drosophila Ovarian Stem Cells

Cynthia Vied, Amy Reilein, Natania S. Field, Daniel Kalderon

The Drosophila ovary and germarium, bab1-GAL4 expression in larval ovaries, and the screening strategy. The Drosophila ovary and germarium, bab1-GAL4 expression.

Volume 16, Issue 23, Pages (December 2006)

Volume 9, Issue 5, Pages (November 2005)

Vertebrate Development: Wnt Signals at the Crest

Germline Stem Cell Heterogeneity Supports Homeostasis in Drosophila

Michael J. Fairchild, Lulu Yang, Katharine Goodwin, Guy Tanentzapf

Todd Nystul, Allan Spradling Cell Stem Cell

Stem Cells: Keeping BMP Signaling Local

Wolbachia Protein TomO Targets nanos mRNA and Restores Germ Stem Cells in Drosophila Sex-lethal Mutants Manabu Ote, Morio Ueyama, Daisuke Yamamoto Current.

Volume 20, Issue 1, Pages (January 2011)

Volume 1, Issue 6, Pages (December 2013)

Volume 1, Issue 4, Pages (October 2007)

Epigenetic Regulation of Stem Cell Maintenance in the Drosophila Testis via the Nucleosome-Remodeling Factor NURF Christopher M. Cherry, Erika L. Matunis

Candidates function in the soma to control GSC and niche cap cell number during larval-pupal transition. Candidates function in the soma to control GSC.

Ismail Osman, Jun Wei Pek Stem Cell Reports

Cortical NuMA enrichment upon Plk1 inhibition is dynein independent.

CK2 is required for early cell divisions in C. elegans embryos

Anterior escort cells are required for germline stem cell maintenance.

Arterial Notch signaling is not required for artery patterning.

Fig. 4. E-cadherin expression level affects monomer dynamics.

Volume 21, Issue 1, Pages (January 2011)

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Alterations in Notch signaling affect the frequency of intermediate and main IHBD branches. Alterations in Notch signaling affect the frequency of intermediate.

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Expression of Shh-Gli target genes in astrocyte progenitors.

Embryonic corneal defects in E18.5 AP-2β NCC KO mutant embryos.

Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos at E12.5. Altered progenitor domain marker protein expression in ShhΔFP/ΔFP embryos.

Hipk induces cell spreading.

Ectopic sprouting and proliferation in Rbpj veins.

Candidates function in the soma during development to control GSC and niche cap cell numbers. Candidates function in the soma during development to control.

Fig. 6. Localization of Sma during growth and migration

Α-Catenin DKO cardiomyocytes exhibit increase proliferation on stiff ECM. (A) Representative images of neonatal cardiomyocytes cultured on different PA-FN.

Adult cardiomyocyte renewal via deletion of Hippo pathway genes.

Hypothetical model of HvAP2 control of stem elongation.

Loss of serotonergic markers after Smed-pitx RNAi is not due to stem cell-based turnover. Loss of serotonergic markers after Smed-pitx RNAi is not due.

Fig. 3. Immunostaining for neural differentiation makers of the stage 29 optic cup.Immunoreactive signals are shown in green or red. Immunostaining for.

Axial organisation of gastruloids.

Study of the specific miRNA profile during the window of implantation (WOI). Study of the specific miRNA profile during the window of implantation (WOI).

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Abnormally low or high Tj expression causes a decrease in DEcad expression and shg enhancer activity. Abnormally low or high Tj expression causes a decrease.

Loss or increase of Tj causes a reduction in Slbo expression.

Sox2 regulates dental epithelial stem cell proliferation and differentiation. Sox2 regulates dental epithelial stem cell proliferation and differentiation.

The actin organization and N-cadherin dynamics in migrating cells.

Fig. 2. Tbx1 lineage is largely complementary to the NSD

Selfish Stem Cells Compete with Each Other

Ectopic Pax7 increases MyoD expression in Pax7-null myogenic cells.

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Peroxisome speeds were slower in patient and control cells.

Epigenetic Regulation of Stem Cell Maintenance in the Drosophila Testis via the Nucleosome-Remodeling Factor NURF Christopher M. Cherry, Erika L. Matunis

Integrin-mediated signaling is required for PFC maturation.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Fig. 8. Expression of other genomic-clustered Chrn subunits in the mesodiencephalon.(A) Schematic representation illustrating the assembly of the Chrnb4,

Effects of paclitaxel and vinblastine.

BCL-3 regulates expression of stemness-associated Wnt targets.

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Vps36 regulates the accumulation of Smo and Hh signaling activity.

PTP1B promotes persistent migration.

The “Skinny” on Wnt Signaling in Stem Cells

Volume 22, Issue 6, Pages (March 2012)

Presentation transcript:

Wnt6 maintains anterior escort cells to promote GSC survival. Wnt6 maintains anterior escort cells to promote GSC survival. (A) C587Gal4 expression of the cell-cycle indicator FUCCI identified two distinct cell populations: the anterior region contained exclusively E2F1-EGFP-positive green cells that are quiescent in G1 phase, whereas the posterior regions contained cycling cells expressing CycB-nls-mRFP (red in S phase, or yellow in M/G2 phases). (B) Wnt6 RNAi significantly reduced the number of both cell populations. (C) Quantification of E2F1-EGFP-positive and CycB-nls-mRFP-positive escort cells as shown in A,B. ***P<0.001 (Student's t-test). Error bars represent s.e.m. (D) Model for how cap cells organize the germline stem cell niche in the fly germarium. Cap cells secrete Wnt6 ligands that act on abutting anterior escort cells to activate Wnt signaling, which is essential for their survival. Anterior escort cells function as an integral component of the GSC niche by promoting BMP signaling in GSCs and DE-cadherin-mediated anchorage of GSCs within the niche. Xiaoxi Wang, and Andrea Page-McCaw Development 2018;145:dev158527 © 2018. Published by The Company of Biologists Ltd