BRAF Kinase Gene V599E Mutation in Growing Melanocytic Lesions

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BRAF Kinase Gene V599E Mutation in Growing Melanocytic Lesions Robert Loewe, Harald Kittler, Gottfried Fischer, Ingrid Faé, Klaus Wolff, Peter Petzelbauer Journal of Investigative Dermatology Volume 123, Issue 4, Pages 733-736 (October 2004) DOI: 10.1111/j.0022-202X.2004.23402.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 DELM pictures from four representative melanocytic lesions. Panels 1a–4a show the lesions at the initial visit; 1b–4b show the respective follow-up pictures. (1) Melanoma with structural changes (arrowheads), as an atypical pigment network, irregular streaks and regression structures after 9 mo. (2) Melanoma with extensive asymmetrical growth within 13 months (arrowheads). (3) Nevus with no apparent changes within the follow-up period of 14 months. (4) Nevus, which increased in size during a 9 mo follow-up. Scale bar=1 mm. Journal of Investigative Dermatology 2004 123, 733-736DOI: (10.1111/j.0022-202X.2004.23402.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 BRAF genotyping of melanocytic lesions. (a) The photograph shows results from amplification reactions with primer pairs detecting the mutated allele (lanes 1–4) or the wild-type (lanes 5–8) of the BRAF gene. In lanes 1 and 5, SKMel-28 cells (containing both the mutated and wildtype allele) were amplified as a positive control, in lanes 2 and 6, water was used as a negative control. In lanes 3 and 7, a heterocygous sample from a melanocytic lesion containing both mutated and wild-type alleles and in lanes 4 and 8, a patient sample containing only wild-type alleles are shown. (b, c) Sequence electropherograms of two different samples are shown. Nucleotide 300 in b is a “W” (“A” and “T” according to the IUB code), indicating a heterozygous sample with one wild-type and one mutated allele, whereas in c, nucleotide 300 is a “T”, indicating a wild-type sample. Journal of Investigative Dermatology 2004 123, 733-736DOI: (10.1111/j.0022-202X.2004.23402.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions