TT‐seq analysis of immediate response to T‐cell stimulation

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TT‐seq analysis of immediate response to T‐cell stimulation TT‐seq analysis of immediate response to T‐cell stimulation AExperimental design. RNA in cells was labeled with 4‐thiouridine (4sU) for consecutive 5‐min intervals. Total and 4sU‐labeled RNA was extracted before T‐cell stimulation and 5, 10, and 15 min after T‐cell stimulation and subjected to deep‐sequencing.B, CExemplary genome browser views for (B) an upregulated mRNA (JUN) and (C) a downregulated mRNA (NKX3‐1). Blue coverage: TT‐seq data for 0, 5, 10, and 15 min after stimulation; gray coverage: total RNA‐seq data for 0, 5, 10 and 15 min after stimulation. TSS: transcription start site, TTS: transcription termination site, pA: poly‐adenylation site. Margaux Michel et al. Mol Syst Biol 2017;13:920 © as stated in the article, figure or figure legend