Changes in regulatory B-cell levels in bone marrow, blood, and sputum of patients with asthma following inhaled allergen challenge John-Paul Oliveria,

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Presentation transcript:

Changes in regulatory B-cell levels in bone marrow, blood, and sputum of patients with asthma following inhaled allergen challenge John-Paul Oliveria, PhD, Amani I. El-Gammal, MD, Michelle Yee, BSc, Caitlin D. Obminski, BSc, Tara X. Scime, BSc, Richard M. Watson, BSc, Karen Howie, BSc, Paul M. O'Byrne, MB, Roma Sehmi, PhD, Gail M. Gauvreau, PhD Journal of Allergy and Clinical Immunology Volume 141, Issue 4, Pages 1495-1498.e9 (April 2018) DOI: 10.1016/j.jaci.2017.11.013 Copyright © 2017 The Authors Terms and Conditions

Fig 1 The blood levels of (A) CD19+ B cells, (B) CD19+FoxP3+ B cells, and (C-F) Breg-cell phenotypes (open bars) and proportions of Breg cells expressing FoxP3 (hatched bars) in patients with asthma with allergies (n = 11), patients without asthma with allergies (n = 7), and healthy controls (n = 7). *P < .05. Journal of Allergy and Clinical Immunology 2018 141, 1495-1498.e9DOI: (10.1016/j.jaci.2017.11.013) Copyright © 2017 The Authors Terms and Conditions

Fig 2 A, The frequency of CD19+FoxP3+ B cells expressed as a percentage of CD19+ B cells in bone marrow (n = 10), blood (n = 10), and sputum (n = 8) of subjects with asthma with allergies at baseline, 7 hours, and 24 hours after allergen (gray bars) and diluent (white bars) inhalation challenge. B, The frequency of CD5+FoxP3+ Breg cells expressed as a percentage of CD19+ B cells in bone marrow (n = 10) of subjects with asthma with allergies at baseline and 24 hours after inhalation challenge and blood (n = 10) of subjects with asthma with allergies at baseline, 7 hours, and 24 hours after inhalation challenge. C, IL-4–induced intracellular expression of FoxP3 and IL-10 of CD19+ B cells and CD5+ Breg cells in CD19+ B cells purified from blood of subjects with asthma with allergies, where each symbol represents a single subject (n = 6). *P < .05. Journal of Allergy and Clinical Immunology 2018 141, 1495-1498.e9DOI: (10.1016/j.jaci.2017.11.013) Copyright © 2017 The Authors Terms and Conditions

Fig E1 Representative gating strategy for regulatory B-cell phenotypes with isotype controls. (A) All cells gate, (B) B cell gate, (C) CD5+ gate, (D) CD5+FoxP3+ isotype control gate, (E) CD5+FoxP3+ Breg gate, (F) CD1d+ gate, (G) CD1d+CD5+ Breg gate, (H) CD24+ gate, (I) CD24+CD27+ Breg gate, and (J) CD24+CD38+ Breg gate. Journal of Allergy and Clinical Immunology 2018 141, 1495-1498.e9DOI: (10.1016/j.jaci.2017.11.013) Copyright © 2017 The Authors Terms and Conditions

Fig E2 The frequency of CD19+ B cells expressed as a percentage of lymphocytes in (A) bone marrow (n = 10), (B) blood (n = 10), and (C) sputum (n = 8) of subjects with asthma with allergies at baseline, 7 hours, and 24 hours after inhalation challenge. The frequency of FoxP3+ Breg-cell phenotypes expressed as a percentage of CD19+ B cells in (D) bone marrow (n = 10) of subjects with asthma with allegies at baseline and 24 hours after inhalation challenge and (E) blood (n = 10) at baseline, 7 hours, and 24 hours after allergen (gray bars) and diluent (white bars). *P < .05. Journal of Allergy and Clinical Immunology 2018 141, 1495-1498.e9DOI: (10.1016/j.jaci.2017.11.013) Copyright © 2017 The Authors Terms and Conditions

Fig E3 IL-4–induced intracellular expression of (A) FoxP3 and (B) IL-10 of CD24+CD27+ Breg cells in CD19+ B cells purified from blood of subjects with asthma with allergies, where each symbol represents a single subject (n = 6). *P < .05. Journal of Allergy and Clinical Immunology 2018 141, 1495-1498.e9DOI: (10.1016/j.jaci.2017.11.013) Copyright © 2017 The Authors Terms and Conditions