Fig. 8. p85β regulates active Cdc42 and Rac localization to deep adhesions.(A) NIH3T3 cell lines expressing p85α or p85β were incubated without serum (2 h),

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Fig. 8. p85β regulates active Cdc42 and Rac localization to deep adhesions.(A) NIH3T3 cell lines expressing p85α or p85β were incubated without serum (2 h), then activated with PDGF (50 ng/ml) for the times indicated. p85β regulates active Cdc42 and Rac localization to deep adhesions.(A) NIH3T3 cell lines expressing p85α or p85β were incubated without serum (2 h), then activated with PDGF (50 ng/ml) for the times indicated. A subset of cells was transfected with Myc-Cdc42. Cell extracts were incubated with purified bacterially bait (GST-rhotekin-RBD for RhoA, GST-Pak1 for Rac/Cdc42, GST-NWASP for Cdc42). As a positive control, NIH3T3 cells were transfected with V12-Cdc42. Total GTPase levels and pulled-down GTP-forms were analyzed in WB (using anti-Myc Ab for Cdc42). The graphs show normalized signal intensity (mean ± s.d.; n = 3) (AU). (B) BLM cells were transfected with control or p85β siRNA in combination with CFP-CRIBPak1 (48 h). The figure shows a representative image in three sequential z-sections. Arrowheads indicate cell adhesions that colocalize with active CFP-CRIB, some organized as rosettes. Bar = 12 µm. (C) Reduction of p85β levels by siRNA transfection as analyzed in WB. Graphs show the percentage of paxillin positive adhesions containing CRIB signal and the percentage of cells showing adhesions of >3 µm depth. * P <0.05 ** P <0.01; Student's t-test. (D) When tumors augment p85β expression, cell adhesions increase their p85β/p110 content (in complex with FAK). This p85β increase, in the presence of growth factors, enables the local accumulation of GTP-Cdc42/Rac at cell adhesion and the generation of a z-axis F-actin core, necessary for invadopodium formation. Ariel E. Cariaga-Martínez et al. Biology Open 2014;bio.20148185 © 2014. Published by The Company of Biologists Ltd