Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. Enterocytes fail to migrate consistently towards the villus tip.

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Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. (A) Time-lapse movie of an intestinal villus within a tissue explant using multiphoton fluorescence. The red box indicates the zoomed region of interest shown in the remaining panels. Ten minutes elapse between frames. Red arrowheads indicate the initial position of a moving cell within the tissue. Green arrowheads indicate the position of the moving cell in the current frame. This cell moves continuously towards the villus tip. Scale bar: 10 μm. (B,C) Relative distance of labelled epithelial cells from the villus tip over time. The black line represents the pattern of motion expected based on previous experiments, assuming constant migration towards the villus tip at a rate of 100 μm per day. Each coloured line indicates the distance from the villus tip of a single representative cell over time. Cell migration seemed to be discontinuous and multidirectional. (B) Cell migration in tissue from wild-type mice. Despite irregularity in cell migration patterns, wild-type cells exhibited a net movement towards the villus tip over time. (C) Cell migration in tissue from APCMin/+ mice. Cells in APCMin/+ tissue, although still dynamic, did not follow as clear a path towards the villus tip as those in wild-type tissue. Scott A. Nelson et al. Dis. Model. Mech. 2012;5:940-947 © 2012. Published by The Company of Biologists Ltd