Catalytic site II mutants of ABCE1 show a dominant negative growth effect. Catalytic site II mutants of ABCE1 show a dominant negative growth effect. In.

Slides:

Advertisements

Similar presentations

Genomes and mutagenesis. Figure 9.15 The Ames test relies on a mutant bacterial strain that is defective in hisG. - Cannot grow on a medium lacking histidine.

Advertisements

Molecular Cell Biology of the Yeast Saccharomyces cerevisiae Lecture I: Biology, Genetics, Genomics and Proteomics Zhang Yi, National Institute of Biological.

Genetics and Genomics Forward genetics Reverse genetics

BISC 220 Lab—Series 2 Protein Transport through the Secretory Pathway

Sergi Puig, Sandra V. Vergara, Dennis J. Thiele Cell Metabolism

BRC Science Highlight WRINKLED1, a key regulator of oil biosynthesis, also affects hormone homeostasis Objective WRINKLED1 (WRI1) is a key transcriptional.

by Kumar Kotlo, Douglas E. Hughes, Victoria L. M

Polina D. Kehayova, David R. Liu Chemistry & Biology

ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome splitting. ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome.

HIS-24 regulates expression of infection-inducible genes.

Protein purification and quality control.

Functional mutants of ABCE1 are potent ribosome splitting factors.

Relationship between Genotype and Phenotype

Volume 19, Issue 6, Pages (September 2005)

Silencing in Yeast rDNA Chromatin

Two nucleotide-binding sites of ABCE1 act functionally asymmetric.

Volume 11, Issue 3, Pages (March 2003)

(A-B) Expression of dominant negative p73. (C) Knockdown of p73

Sergi Puig, Sandra V. Vergara, Dennis J. Thiele Cell Metabolism

Purification of budding yeast cohesin and its loader.

Parul Mishra, Daniel N.A. Bolon Molecular Cell

Ssu72 is required for polymerase α activation

Volume 8, Issue 5, Pages (February 1998)

Histone-like TAFs Are Essential for Transcription In Vivo

Young-Hee Cho, Sang-Dong Yoo, Jen Sheen Cell

Volume 99, Issue 7, Pages (December 1999)

Direct role of HIS-24 and HPL in control of microbial resistance.

Direct role of HIS-24 and HPL in stress response.

Volume 12, Issue 5, Pages (August 2015)

Volume 29, Issue 3, Pages (February 2008)

HDAC11 regulates CCL2 expression by recruiting PU.1.

Secretion efficiency of recombinant laminin-111, laminin-511, and their EQ mutants. Secretion efficiency of recombinant laminin-111, laminin-511, and their.

Overrepresentation or underrepresentation of amino acids at different positions in ErmBL. Overrepresentation or underrepresentation of amino acids at different.

NsiR4 expression is mediated through an NtcA-activated promoter.

Relative expression levels of known mRNA selected as points of reference. Relative expression levels of known mRNA selected as points of reference. mRNA.

ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor aPelota. ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor.

Cellular localization of r-proteins during repression of a 40S or a 60S r-protein gene. Cellular localization of r-proteins during repression of a 40S.

Statistics of cleavage sites and mutant-enriched sites.

%E0%A4%B8%E0%A5%8D%E0%A4%9F%E0%A5%8B%E0%A4%B0%E0%A5%80%E0%A4%9C/%E0 %A4%B0%E0%A5%87%E0%A4%AE%E0%A5%8D%E0%A4%AF%E0%A4%BE-

ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome splitting. ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome.

ATPase activity of the ABCE1 disengagement and mixed mutants.

Quantification method for the nucleotide occlusion assay.

Summary of RNA FISH quantifications.

Protein purification and quality control.

Both nucleotide-binding sites must close for efficient 70S splitting.

GATC sites where methylation changes over time correspond to a change in gene expression in a dam mutant. GATC sites where methylation changes over time.

Molecular mechanism of ribosome recycling by ABCE1.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

SHU2 mutants have phenotypes similar to the Rad51 paralogs.

Relationship between Genotype and Phenotype

Fig. 4. Autotoxicity triggered by withdrawal of antitoxin inducer in Synechocystis sp. Autotoxicity triggered by withdrawal of antitoxin inducer in Synechocystis.

CREB1 promotes the induction of endogenous ERα target genes.

A proximal region of the G6Pase catalytic subunit promoter is sufficient for maximal basal reporter gene expression in HIT cells. A proximal region of.

ESCRT complexes are required for efficient consumption of LDs

ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor aPelota. ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor.

Functional promoter activity of the human COX-2 gene in HMCs

ABCE1 occludes two nucleotides during 70S splitting.

The effect of Bos1 mutations on yeast growth.

Acne Inversa Caused by Missense Mutations in NCSTN Is Not Fully Compatible with Impairments in Notch Signaling Xulun Zhang, Sangram S. Sisodia Journal.

Oleksi Petrenko, Ute M. Moll Molecular Cell

Volume 3, Issue 2, Pages (February 2013)

%E0%A4%B8%E0%A5%8D%E0%A4%9F%E0%A5%8B%E0%A4%B0%E0%A5%80%E0%A4%9C/%E0 %A4%B0%E0%A5%87%E0%A4%AE%E0%A5%8D%E0%A4%AF%E0%A4%BE-

Transcriptional Termination Factors for RNA Polymerase II in Yeast

Targeting DCLK1 by miRNA-137.

GPC3 promotes hepatocellular carcinoma growth by stimulating the canonical Wnt pathway (A-B) GPC3 induces the stabilization of cytoplasmic β-catenin. GPC3.

Transcriptional activity and growth of mutant ER in a breast cancer cell line. Transcriptional activity and growth of mutant ER in a breast cancer cell.

Production of ade2Δ/ade2Δ mutants by the Candida CRISPR system.

The Engagement of Sec61p in the ER Dislocation Process

DNSP16 mutant MERS-CoV infection produces minimal changes in early host responses. dNSP16 mutant MERS-CoV infection produces minimal changes in early host.

BRCA1 sequence variants classified as pathogenic do not restore HR

Presentation transcript:

Catalytic site II mutants of ABCE1 show a dominant negative growth effect. Catalytic site II mutants of ABCE1 show a dominant negative growth effect. In minimal medium lacking histidine (–HIS) and 5-FOA, yeast cells express WT and mutant ABCE1, both under the control of the endogenous promoter. Toxic ABCE1 mutants E493A, E493Q (site II), E247A/E493A (both sites), S223R (site II), and S469R (site I) poise the translation apparatus and cause a dominant negative growth defect even in the presence of the WT gene. In the presence of 5-FOA, the URA3 selection marker leads to loss of the pRS426 vector carrying the WT ABCE1 gene and yeast cells can only survive with the ABCE1 on pRS423. Here, only the cells that rely on the catalytic site I variant E247A are viable. Cells that depend on E247Q show an impaired growth. Elina Nürenberg-Goloub et al. LSA 2018;1:e201800095 © 2018 Nürenberg-Goloub et al.