Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin.

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Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin. Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin. (A) Western blot analysis of Drosophila head (lane 1), cockroach brain (lane 2) and cockroach salivary gland (lane 3) with anti-synapsin. A broad band with an electrophoretic mobility of about 80 kDa is intensely labelled in all preparations. In Drosophila, an additional synapsin isoform is detected at about 140 kDa. (B) In vitro binding of the synapsin-positive cockroach protein to actin filaments. An extract of cockroach brain was incubated with F-actin; actin filaments were then pelleted by high-speed centrifugation. In the absence of F-actin, the anti-synapsin-positive proteins remain entirely in the supernatant (S), whereas in the presence of F-actin, a substantial fraction of the anti-synapsin-positive proteins is detected in the pellet (P) together with F-actin. The actin-binding protein α-spectrin provides a positive control, and tubulin a negative control. Otto Baumann et al. J Exp Biol 2004;207:2565-2575 © The Company of Biologists Limited 2004