eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots

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Fig. 4. eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots. eb1a-2 eb1b-3 double-mutant plants display skewed and shorter roots. (A) Typical pictures illustrating root skewing and root length for control and eb1a-2 eb1b-3 double-mutant plants (both expressing 35S::GFP:TuA6). (B) Measurement of the root-skewing angle for both genotypes, n=89 for the control and n=84 for the double mutant. (C) Measurement of the root length for both genotypes, n=93 for the control and n=76 for the double mutant. Asterisks represent statistically significant differences (Kruskal–Wallis, α=0.01). Bars indicate s.e.m. (D) Root apical meristem from Col-0 and (E) root apical meristem from eb1a-2 eb1b-3 double-mutant plants after 7 days of growth on 0.3% agar medium. (F) Box plot showing the root apical meristem length in Col-0 and double mutant, measured from the quiescent center to the beginning of the elongation zone. Crosses represent the mean, bars represent the median and whiskers indicate minima and maxima. n=17 for Col-0 and n=14 for the double mutant. Difference is not significant according to a t-test with α=0.05. Scale bar: 50 μm. Arthur T. Molines et al. Biology Open 2018;7:bio030510 © 2018. Published by The Company of Biologists Ltd