Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and.

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Fig. 1. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and stained with the indicated antibodies. γ-Tubulin localizes in close proximity to centriole walls in interphase but within an extended PCM meshwork in mitosis.U2OS cells were fixed and stained with the indicated antibodies. Representative 3D-SIM optical mid-sections through centrosomes are shown for different cell cycle stages (Scale bars 0.5 µm). (A) γ-Tubulin and NEDD1 were stained in interphase and mitosis, respectively. Arrows point to regions of signal overlap. Correlative widefield image (WF) of green (NEDD1) and Cy5 (CP110) channels and schematic illustration of centriole orientation are shown to the right of the interphase cell. Overview panel shows centrosome separation (Scale bar 5 µm, lower panel). (B) γ-Tubulin localization in interphase and mitosis is shown relative to Pericentrin, Cep215 and Cep192. Arrowheads indicate mutually exclusive staining. Katharina F. Sonnen et al. Biology Open 2012;1:965-976 © 2012. Published by The Company of Biologists Ltd