Fig. 7. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo.

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Fig. 7. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo. (A) Gating strategy of flow cytometric analysis. CD45−c-Kit−CD71+/−Ter119+ defines terminally-differentiating erythroid cells and mature red blood cells. (B) Graph showing the number of terminally-differentiating erythroid cells and mature red blood cells in mice treated with DMSO, the SCF inhibitor, the IGF-1R inhibitor or both, two hours after injection (n = 3). (C) Relative expression (RQ) of erythroid genes such as Gata1, Klf1 and β-major globin mRNAs in CD45− cells was examined by real-time PCR. CD45− cells from mice injected with DMSO served as controls (n = 3). Data are means±standard deviation (SD). *P<0.05. Keai Sinn Tan et al. Biology Open 2015;bio.201410686 © 2015. Published by The Company of Biologists Ltd