Fig. 1. Ovarian cancer spheroids can bud from a monolayer

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Fig. 1. Ovarian cancer spheroids can bud from a monolayer Fig. 1. Ovarian cancer spheroids can bud from a monolayer.A2780 cells were grown as a monolayer in media containing serum. Ovarian cancer spheroids can bud from a monolayer.A2780 cells were grown as a monolayer in media containing serum. (A–C) Phase contrast images of the spheroid budding process. (A) Representative spheroid budding from the monolayer at a site of vertical expansion. (B) Representative spheroid that had detached from the monolayer and was floating freely in the culture media. (C) Representative spheroid plated in the presence of serum, which had re-adhered to the culture dish and formed a new monolayer. (D,E) Fluorescence images of a budding spheroid stained with DRAQ5 to visualize cell nuclei and imaged while alive. The same spheroid is shown from the top, or X–Y view (D), and the side, or Y-Z view, which was digitally reconstructed from confocal image stacks (E). Scale bars: 30 μm. Jillian C. Pease et al. Biology Open 2012;bio.2012653 © 2012. Published by The Company of Biologists Ltd