Polymorphism T→C (−34 bp) of gene CYP17 promoter in Greek patients with polycystic ovary syndrome  Evanthia Diamanti-Kandarakis, M.D., Ph.D., Michael I Bartzis, B.Sc., Evangelia D Zapanti, M.D., Ph.D., Giovanna G Spina, M.D., Fanny A Filandra, M.D., Thomais C Tsianateli, M.D., Angeliki T Bergiele, M.D., Chryssa R Kouli, M.D.  Fertility and Sterility  Volume 71, Issue 3, Pages 431-435 (March 1999) DOI: 10.1016/S0015-0282(98)00512-3

FIGURE 1 Electrophoresis on a 3% agarose gel of the MspA1 I–digested polymerase chain reaction (PCR)-amplified 459-bp fragment from gene CYP17 promoter, which contains the site (−34bp) where the T→C transition occurs, creating the A2 allele. Lanes 2 and 3: Heterozygous A2 carriers. Lane 4: Individual homozygous for the A2 allele. Lane 5: Noncarrier of the A2 allele. Lane 6: Individual with intact PCR product (nonincubated with MspA1). Lane 8: pGL-2 basic DNA digested with MspA1 I. The sizes of the fragments were estimated using the ladder PhiX174 DNA Hae III digest (lanes 1 and 7) and Lambda DNA Hind III digest (lane 9). The gel was stained with ethidium bromide to permit visualization of DNA bands on UV irradiation. Fertility and Sterility 1999 71, 431-435DOI: (10.1016/S0015-0282(98)00512-3)

FIGURE 2 Mean serum total T levels in the three CYP17 polymorphism genotype groups. There were 17, 29, and 4 individuals in the A1A1, A1A2, and A2A2 groups, respectively. Mean (SD) serum total T levels for the three groups were as follows: A1A1, 75.7 ± 32.2 ng/dL; A1A2, 77.8 ± 29.9 ng/dL; and A2A2, 87 ± 2.8 ng/dL. P = not significant (A1A1 versus A1A2, A1A1 versus A2A2, and A1A2 versus A2A2). Fertility and Sterility 1999 71, 431-435DOI: (10.1016/S0015-0282(98)00512-3)