Beer anaphylaxis Journal of Allergy and Clinical Immunology

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Presentation transcript:

Beer anaphylaxis Journal of Allergy and Clinical Immunology Silvia Fernández-Anaya, MD, Jesus F. Crespo, MD, PhD, Julia R. Rodríguez, MD, Pilar Daroca, MD, Eva Carmona, MD, Lys Herraez, MD, Arancha López-Rubio, MD Journal of Allergy and Clinical Immunology Volume 103, Issue 5, Pages 959-960 (May 1999) DOI: 10.1016/S0091-6749(99)70449-5 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 IgE antibody reactivity to barley-made beer allergens by immunoblotting. Proteins in beer were resolved by means of SDS-PAGE and transferred to PVDF. Five-millimeter strips from each blot were tested for reactivities to serum IgE from patients with beer anaphylaxis. Lane A, Molecular weight markers; lane B, staining for proteins by amido black; lanes C through E, sera from patients 1, 2, and 3, respectively; lanes F through I, IgE inhibition experiments performed with barley extract. A serum pool from patients 1, 2, and 3 was preincubated with 1000 μg/mL, 500 μg/mL, and 10 μg/mL of barley extract (lanes F, G, and I ). PVDF-blotted beer proteins were incubated with this solution, and immunoblotting was performed. Journal of Allergy and Clinical Immunology 1999 103, 959-960DOI: (10.1016/S0091-6749(99)70449-5) Copyright © 1999 Mosby, Inc. Terms and Conditions