TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. (A) SIRT3 mRNA levels in tubular cells incubated with 100 ng/ml TWEAK. Mean±s.e.m. of three independent experiments. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (B) Total protein levels of SIRT3 in MCT cells incubated with 100 ng/ml TWEAK for different periods of time. Mean±s.e.m. of three independent experiments. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (C) Kidney SIRT3 mRNA levels in mice treated with TWEAK. Mean±s.e.m. of five animals per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (D) Kidney SIRT3 protein levels in mice treated with TWEAK for different times. Mean±s.e.m. of five animals per group. *P<0.05 vs control (non-parametric Mann–Whitney U-test). (E) SIRT3 mRNA levels were tested in tubular cells incubated with 100 ng/ml TWEAK with or without 50 mM crotonate for 6 h. Data from three independent experiments and expressed as mean±s.e.m. *P<0.05 vs vehicle control; #P<0.05 vs vehicle TWEAK (non-parametric Mann–Whitney U-test). (F) Total SIRT3 protein levels in tubular cells incubated with 100 ng/ml TWEAK with or without 50 mM crotonate for 48 h. Data from four independent experiments and expressed as mean±s.e.m. *P<0.05 vs vehicle control, #P<0.05 vs vehicle TWEAK (non-parametric Mann–Whitney U-test). Olga Ruiz-Andres et al. Dis. Model. Mech. 2016;9:633-645 © 2016. Published by The Company of Biologists Ltd