Sajany Gas Vortex Gradientless Bioreactor Сreation of highly efficient universal modular biotechnological complexes of a new type based on gas vortex gradientless bioreactors Joint Stock Company office 403, building 8, Nikolaeva str., Novosibirsk Tel/fax: +7 (383) ; ,

The factors affecting the vital capacity of cultivated cells and microorganisms (temperature regime, breath, nutrition, metabolites, mechanical impact)

Mechanical mixing Gas vortex mixing

Traditional bioreactor: mechanical mixing

Gas vortex gradientless bioreactor: gas vortex mixing

Characteristics of gas vortex gradientless bioreactors Extremely low power consumption, i.e times less compared to bioreactors with mechanical stirrer Cultivation of cells which are poorly reproduced in well-known types of bioreactors High surface mass exchange rate (KL 1/ hour) Efficient mixing of especially viscous fluids Absence of local overheating areas affecting the enzymes activity Constant operational parameters when filled with a suspension by 15%-90% of the vessel volume Gas vortex serves as an efficient foam breaker Bioreactors hydrodynamics, practically, does not depend on the liquid level inside. The bioreactor is easy to scale-up

Some Cultivation Results (Without Medium Substitution) Besides, chicken embryo fibroblasts, VERO, hybrid A 4 С 5 cells (a hybrid of porcine kidney cell and lymphocyte), human thymus cells (T-5) and Aristolochia manshunensis cells, Aspergillus awamori, Fusarium moniliforme and many others were successfully cultivated.

Important advantages of gas vortex gradientless bioreactors Universality – capability to operate successfully practically on all types of cells (including embryonic and hybrid ones) and microorganisms Full repeatability (scalability) of laboratory results at industrial application of developments High economic efficiency in commercial use

Industrial implementation of innovative products and technologies ? ! ? ! Traditional bioreactor Gradientless bioreactor New product

Application of gas-vortex bioreactors for emergency responce Epidemics, pandemics Emergency eliminations Global warming Technogenic impact Bioterrorism Global warming Technogenic impact Bioterrorism Appearance of diseases unknown before Express manufacturing of any type vaccines in sufficient amounts using gas-vortex bioreactors Developments of required vaccines

Conception of highly efficient universal modular biotechnological enterprises of a new type Highly efficient universal modular biotechnological enterprise of a new type consists of: fixed highly economical head block of the universal gas vortex gradientless bioreactors variable modular tail block responsible for the processing of biological products Modules of the tail block can be modified and enlarged depending on the processed biological product which is got from block of gas vortex gradientless bioreactors

Universal highly efficient biotechnological modular production facilities 750 L 10 L 30 L 100 L 300L 300 L 10 L 30 L 100 L 300 L Fermentative unit with traditional bioreactors Fermentative unit with gas-vortex bioreactors 750 L 10 L

Factors that highlight high profitability of the new production facility Minor initial investments as a results of Reduction of the number of bioreactors in the technological chain Making communication pipelines simpler and shorter Reduction of a total production costs (electric power, steam, detergents, area, payment schedule etc.) Universality of the facilities, i.e. feasibility of successful operation with producers of different types Financial stability of the enterprise as a result of rapid and low- investment implementation of technologies for manufacture of new biotechnological products Technological effectiveness Augmentation of the biomass yield Reduction of losses during cultivation

Field of Application Production of medical products (including products using very sensitive embryonic, hybrid and other cells); Production of a broad range of microbiological preparations for agriculture and veterinary medicine; Production of polysaccharides and oil destructors for oil-producing industry; Manufacturing of products for food industry (treacle, glucose syrups, ferments, food supplements and etc.); Production of surface-active agents and ferments for chemical industry; Carrying out research efforts of different types.

Promptly-built modular biotechnological (pharmaceutical) industrial complexes of a new type Broad application of gas- vortex bioreactors and the creation of biotechnological industrial complexes of the new type on their basis is one of the breakthrough trends in the field of industrial biotechnology.

Gas Vortex Gradientless Bioreactor is an apparatus of new generation having no analogues in the whole world in terms of the method of mixing patents of USA, Japan and 6 European countries Gas-Vortex Gradientless Bioreactor is acknowledged by experts to be one of the most promising creations in the sphere of high technologies in Russia in In III Moscow International Congress «BIOTECHNOLOGY: STATE OF THE ART AND PROSPECTS OF DEVELOPMENT» Gas Vortex Gradientless Bioreactor became the winner in the nomination Equipment and was awarded gold medal at BIOTECH WORLD 2005 International Exhibition.

Thank you office 403, Building 8, Nikolaeva str., Novosibirsk Tel/fax: +7 (383) ; , Sajany Joint Stock Company