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Ondřej Zítka Datum konání: 6.11.2015 Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties Ondřej Zítka Datum konání: 6.11.2015 Investice do rozvoje vzdělávání

Background Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids.

The Ultrafiltration of vesicles

Summary The authors shows that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.

Investice do rozvoje vzdělávání Acknowledgements: Project: Paper: Budování výzkumných týmů a rozvoj univerzitního vzdělávání výzkumných odborníků pro mikro- a nanotechnologie (NANOTEAM) CZ.1.07/2.3/.00/09.0224 Nordin, J.Z., et al., Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine-Nanotechnology Biology and Medicine, 2015. 11(4): p. 879-883. Investice do rozvoje vzdělávání