Both nucleotide-binding sites must close for efficient 70S splitting.

Slides:

Advertisements

Similar presentations

Hydration profiles of GLIC′s pore in WT and Ser6′ mutant simulations.

Advertisements

Checklist for Site Reconnaissance (Sabatini et al_2002)

IRES‐dependent translation is specifically abolished by an IRES point mutation. IRES‐dependent translation is specifically abolished by an IRES point mutation.

Interaction of SRP19 with nuclear transport receptors.

PGAM5 dephosphorylates ASK1.

Both patient 1 and her family members showed a normal physiological AQP2 response to DDAVP. Immunoblotting for AQP2 was performed on urinary exosomes derived.

ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome splitting. ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome.

Protein purification and quality control.

Functional mutants of ABCE1 are potent ribosome splitting factors.

Laura Lancaster, Harry F. Noller Molecular Cell

Phosphorylation of a tyrosine residue in mGluR1a-CT1 by Fyn.

Two nucleotide-binding sites of ABCE1 act functionally asymmetric.

Fátima Gebauer, Marica Grskovic, Matthias W Hentze Molecular Cell

H-NS2 is upregulated in an hns mutant derivative of the strain E

SDS–PAGE and immunoblots of purified IgG for citrullination and carbamylation. a) Representative Coomassie-blue-stained isolated total IgG from patients.

Identification of miR‐499 targets

Purification of budding yeast cohesin and its loader.

GAK phosphorylates Atp1a3 at novel site T705 in HEK 293Ts.

Usp5 sensitivity of S65 ubiquitin mutant chains Structure of ubiquitin in complex with Usp5 (PDB ID: 3IHP). Usp5 sensitivity of S65 ubiquitin mutant chains.

PKA phosphorylates Thr63 and Ser692 to increase HIF-1α stability.

Cohesin-loader−stimulated cohesin loading.

Mechanism of Force Generation of a Viral DNA Packaging Motor

Caitlin M. McDonold, J. Christopher Fromme Developmental Cell

Purification and characterization of budding yeast cohesin and its loader. Purification and characterization of budding yeast cohesin and its loader. (A)

How Integration of Positive and Negative Regulatory Signals by a STAND Signaling Protein Depends on ATP Hydrolysis Emélie Marquenet, Evelyne Richet

Structure, Exchange Determinants, and Family-Wide Rab Specificity of the Tandem Helical Bundle and Vps9 Domains of Rabex-5 Anna Delprato, Eric Merithew,

Volume 113, Issue 1, Pages (July 2017)

Volume 9, Issue 1, Pages (January 2002)

Regulation of RECQ4–MCM10 interaction by CDK phosphorylation.

HDAC11 regulates CCL2 expression by recruiting PU.1.

FRAP analysis of Cdt2 binding to sites of damage in the absence of Cdt1. FRAP analysis of Cdt2 binding to sites of damage in the absence of Cdt1. (A) Quantitative.

Purity of Lnp and its derivatives.

Secretion efficiency of recombinant laminin-111, laminin-511, and their EQ mutants. Secretion efficiency of recombinant laminin-111, laminin-511, and their.

NsiR4 expression is mediated through an NtcA-activated promoter.

Identification of the AlkZ active site.

ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor aPelota. ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor.

Characterization of SYNCRIP-SMN1 methylation–dependent interaction.

Visualization of differential changes between chronic versus acute PDGFRα stimulation. Visualization of differential changes between chronic versus acute.

Recombinant laminins used for in vitro binding assay.

Position of dsRBD3 and dsRBD4 and role of the connecting linker.

The saturation scan database for Ubv.2.1 (A) and Ubv.21.4 (B).

Methylation dependency of SNYCRIP protein interactions.

Statistics of cleavage sites and mutant-enriched sites.

ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome splitting. ATPase activity of ABCE1 is stimulated by 70S and aRF1 during ribosome.

ATPase activity of the ABCE1 disengagement and mixed mutants.

Quantification method for the nucleotide occlusion assay.

Protein purification and quality control.

Catalytic site II mutants of ABCE1 show a dominant negative growth effect. Catalytic site II mutants of ABCE1 show a dominant negative growth effect. In.

GATC sites where methylation changes over time correspond to a change in gene expression in a dam mutant. GATC sites where methylation changes over time.

RECQL4 is a RanGTP dependent MAP

Molecular mechanism of ribosome recycling by ABCE1.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

Tumor phenotyping. Tumor phenotyping. (A) Comparative growth behavior of tumors after grafting R versus VR J2A, H1, and I1 cell lines in independent experiments.

Arginine-to-lysine mutations conferring TRIM22 restriction and lysine-to-arginine mutations causing loss of TRIM22 restriction. Arginine-to-lysine mutations.

Relative growth of transcription factor knockout mutants on different sulfur sources. Relative growth of transcription factor knockout mutants on different.

Comparison of biological replicates for Ery-treated samples.

Endogenous salicylic acid (SA) triggers stomatal closure and immune defense in Atg3bp1 mutants. Endogenous salicylic acid (SA) triggers stomatal closure.

Enrichment of 3-aa motifs upon addition of Ery.

ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor aPelota. ABCE1 splits 70S with the canonical aRF1 and the ribosome rescue factor.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

Volume 20, Issue 3, Pages (November 2005)

Thermodynamic analysis of the interaction between diazaborines and M

ABCE1 occludes two nucleotides during 70S splitting.

SusG LES is required for efficient packing into OMVs

Temporal profiles of eye velocity and neural activity for normal saccades. Temporal profiles of eye velocity and neural activity for normal saccades. Green.

Empty Site Forms of the SRP54 and SRα GTPases Mediate Targeting of Ribosome– Nascent Chain Complexes to the Endoplasmic Reticulum Peter J Rapiejko, Reid.

Translation Initiation from the Ribosomal A Site or the P Site, Dependent on the Conformation of RNA Pseudoknot I in Dicistrovirus RNAs Nobuhiko Kamoshita,

Transplantability of a circadian clock to a noncircadian organism

ChIP-chip analysis of wild-type (WT) and RpoZ-defective mutant RNAP

TRK inhibitor binding to acquired resistance mutations.

Presentation transcript:

Both nucleotide-binding sites must close for efficient 70S splitting. Both nucleotide-binding sites must close for efficient 70S splitting. (A) A minimal set of splitting factors required for active splitting comprises ABCE1, aRF1, and aIF6 from S. solfataricus. All factors were pure and monodisperse as shown by SDS–PAGE (Coomassie), immunoblotting, and SEC. (B) Specific 70S splitting requires aRF1, ABCE1, and AMP-PNP. Results were normalized to the highest splitting ratio for WT ABCE1 with AMP-PNP and aRF1. (C) Traces corresponding to (B) clearly demonstrate an increase of 50S in the presence of WT ABCE1, aRF1, and AMP-PNP. (D) Ribosome splitting is most efficient, when both sites are in a closed, occluded state. Colors as in (B). (E) No ribosomes are actively split by any of the SR “disengagement” mutants, showing that closure of both sites is a prerequisite for ribosome splitting. Colors as in (B). Representative set of three independent experiments. Elina Nürenberg-Goloub et al. LSA 2018;1:e201800095 © 2018 Nürenberg-Goloub et al.