Prolactin gene expression in human ovarian follicular cells

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Presentation transcript:

Prolactin gene expression in human ovarian follicular cells John Y. Phelps, M.D., Elizabeth M. Bugg, M.S., Michael J. Shamblott, Ph.D., Nikos P. Vlahos, M.D., Joe Whelan, M.D., Howard A. Zacur, M.D., Ph.D. Fertility and Sterility Volume 79, Issue 1, Pages 182-185 (January 2003) DOI: 10.1016/S0015-0282(02)04536-3

FIGURE 1 Representative illustration of RT-PCR products from follicular cells of two of the seven patients studied; prolactin gene expression in human ovarian follicular cells as shown by PCR-amplified cDNA, separated in an ethidium bromide–stained 1% agarose gel. Lane 1, 123-bp ladder. Lane 2, patient 1 FC, RT+. Lane 3, patient 1 FC, RT−. Lane 4, patient 2 FC, RT+. Lane 5, patient 2 FC, RT−. Lane 6, pituitary, RT+. Lane 7, pituitary, RT−. Lane 8, kidney, RT+. Lane 9, kidney, RT−. Lane 10, no template, RT+. Lane 11, no template, RT−. FC = follicular cells; RT = reverse transcriptase. Phelps et al. Ovarian prolactin gene expression. Fertil Steril 2003. Fertility and Sterility 2003 79, 182-185DOI: (10.1016/S0015-0282(02)04536-3)