Reduced local immune response with continuous positive airway pressure during one- lung ventilation for oesophagectomy R.J.J. Verhage, J. Boone, G.T.

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Reduced local immune response with continuous positive airway pressure during one- lung ventilation for oesophagectomy R.J.J. Verhage, J. Boone, G.T. Rijkers, G.J. Cromheecke, A.C. Kroese, T.J. Weijs, I.H.M. Borel Rinkes, R. van Hillegersberg British Journal of Anaesthesia Volume 112, Issue 5, Pages 920-928 (May 2014) DOI: 10.1093/bja/aet476 Copyright © 2014 The Author(s) Terms and Conditions

Fig 1 Flowchart of trial design, inclusion, and exclusion. *Exclusions from the control group: extensive pleural adhesions, converted to transhiatal procedure (n=1); insufficient collapse of the right lung, converted to transhiatal procedure (n=2); irresectable tumour (n=1); gastrectomy instead of oesophagectomy (n=1). †Exclusions from the CPAP group: irresolvable hardware issues with robotic system, converted to transhiatal procedure (n=2); extensive pleural adhesions, converted to transhiatal procedure (n=1); irresectable tumour (n=2). British Journal of Anaesthesia 2014 112, 920-928DOI: (10.1093/bja/aet476) Copyright © 2014 The Author(s) Terms and Conditions

Fig 2 Sequence of randomization and sampling. British Journal of Anaesthesia 2014 112, 920-928DOI: (10.1093/bja/aet476) Copyright © 2014 The Author(s) Terms and Conditions

Fig 3 Concentration changes of cytokines in BALF. Longitudinal concentrations of cytokines (a, IL-1α; b, IL-1β; c, IL-6; d, IL-10; and e, TNFα) as change from baseline (preoperative) in the left and right lung of patients with and without CPAP. Data represent the mean (sem) difference of the natural logarithm concentration (Ln) from t1. P-values indicate the difference between the control and CPAP groups at t4. Sample moments: t1, preoperative; t2, 2 h after collapse; t3, 2 h after reinsufflation; t4, postoperative. British Journal of Anaesthesia 2014 112, 920-928DOI: (10.1093/bja/aet476) Copyright © 2014 The Author(s) Terms and Conditions

Fig 4 Concentration changes of chemokines in BALF. Longitudinal concentrations of chemokines (a, MCP-1; b, MIP-1α; c, PARC; and d, IL-8) as change from baseline (preoperative) in the left and right lung of patients with and without CPAP. Data represent the mean (sem) difference of the natural logarithm (Ln) concentration from t1. P-values indicate the difference between the control and CPAP groups at t4. Sample moments: t1, preoperative; t2, 2 h after collapse; t3, 2 h after reinsufflation; t4, postoperative. British Journal of Anaesthesia 2014 112, 920-928DOI: (10.1093/bja/aet476) Copyright © 2014 The Author(s) Terms and Conditions