ER-to-Golgi SNARE complex formation with Bos1 p.Arg196del.

Slides:



Advertisements
Similar presentations
Mitochondria localize to both the front and the rear of neutrophils.
Advertisements

SESN2 expression in lungs from healthy donors, habitual smokers without COPD and individuals with late-stage COPD. (A) Representative western blots of.
Obesity accompanied by increased triacylglyceride deposits in InsP3R mutants leads to starvation resistance. Obesity accompanied by increased triacylglyceride.
Volume 139, Issue 6, Pages (December 2009)
Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants.
Abnormalities of the aortic arch in Hectd1opm/opm mutant embryos.
In silico simulation of the SNARE complexes bearing the two PME mutations. In silico simulation of the SNARE complexes bearing the two PME mutations. Molecular.
Inducible DM1 model displays increased autophagy.
SNARE motif sequence alignment of GOSR2 and its yeast ortholog, Bos1.
VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown.
Effect of chaperonin 60 antisense construct levels on susceptibility to Legionella. Effect of chaperonin 60 antisense construct levels on susceptibility.
Representative histological images of aorta from WT, ApoEm/m and ApoE−/− pigs. Representative histological images of aorta from WT, ApoEm/m and ApoE−/−
OA and related amines modulate motility and length of schistosomula.
C. elegans cell-lineage map and multi-vulva mutants.
X-ray radiographic images showing structural alterations of the joints that are most commonly affected by OA. (A) Normal and (A′) severely affected joint.
TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.
Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. Enterocytes fail to migrate consistently towards the villus tip.
Survival and telomere length of G2tert−/− zebrafish in a p53−/− background. Survival and telomere length of G2tert−/− zebrafish in a p53−/− background.
Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.
Pie charts showing the percentage of genes for selected enriched biological functions and pathways at different time points after hyperthermic seizures.
Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice. Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice.
Obesity affected the metabolic response to stroke.
Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.
Expression data confirm and extend existing knowledge
Factors affecting standardization of rodent impact TBI models.
Table 3. Penetrance of cuticle defects seen in ush2 mutants embryos and in the progeny from rescue experiments (genotypes indicated in the table) with.
Combined treatment with LY and sunitinib rescued hypervascularization.
tert mutant zebrafish can be bred for only one generation.
Treatment of HCMT-CMs with bisoprolol.
Superimposed traces representing the effects of different concentrations of adrenaline on AP characteristics. Superimposed traces representing the effects.
Tamoxifen injections in αMHC-MerCreMer mice induce a loxP-independent DNA damage response, leading to myocardial dysfunction and death. Tamoxifen injections.
Elevated expression of vegfaa in hemangiosarcoma.
Simultaneous perturbations to shuttling result in defects in the Dl gradient and in the sna domain. Simultaneous perturbations to shuttling result in defects.
The effect of TDP-43A315T expression on dendrite spine development.
The mutants exhibit congenital hypothyroidism.
Distribution and extent of expression.
The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.
The feelgood mutation affects craniofacial skeletal development.
Elevation of ERK downstream signaling is associated with tumor formation in the bladder and in papilloma formation. Elevation of ERK downstream signaling.
Rgs16::GFP expression in pancreatic neoplasia.
Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.
Tissue ATP levels measured in the liver, muscle, kidney and pancreas of CDs/y and CDr/y on RD or DD. *P
Increased expression of the drusen-associated genes (ApoE, ApoJ, ApoB and App) and Vegfa in the RPE/retina of Pgc-1α+/− mice under RD and HFD. (A) The.
Expression analysis of periostin in DRG
Systemic infection in Drosophila larvae by septic injury with a fine tungsten needle. Systemic infection in Drosophila larvae by septic injury with a fine.
Food-additive exposure reduces adult fly survival under some feeding conditions. Food-additive exposure reduces adult fly survival under some feeding conditions.
Impact of maternal loading for GALK on survival of larvae to adulthood in the presence versus absence of 200 mM dietary galactose. Impact of maternal loading.
Anti-epileptic drugs with Na+ channel blocking activity improve QTc in individuals with Rett syndrome. Anti-epileptic drugs with Na+ channel blocking activity.
Cross-validation between centres for inbred mice.
Therapeutic delivery of rhIGF-1 reduces the severity of contact hypersensitivity responses. Therapeutic delivery of rhIGF-1 reduces the severity of contact.
Pgc-1α+/− mice show an increased inflammatory response to LPS injection. Pgc-1α+/− mice show an increased inflammatory response to LPS injection. (A) RPE/retina.
Light-dark cycles modulate neurodegeneration in bgm and dbb single mutants. Light-dark cycles modulate neurodegeneration in bgm and dbb single mutants.
V-ATPase and proteins involved in late endosomal protein sorting are required for efficient utilization of LDs during growth resumption. V-ATPase and proteins.
Examples of tumors represented in Tp53Δ11/Δ11 and Tp53Δ11/+ rats.
WASP deficiency produces aberrant F-actin distribution, increased adhesion and downregulation of CD43 during megakaryocytic differentiation. WASP deficiency.
ESCRT complexes are required for efficient consumption of LDs
ER stress and ultrastructural pathology of IECs
Peroxisome speeds were slower in patient and control cells.
Structure of the N-linked core glycan.
Spatial and temporal distribution of autofluorescent (AF) spots and rosettes in rd7 mice. Spatial and temporal distribution of autofluorescent (AF) spots.
Misfolding mutations impair the BRCA1 nuclear aggregation in yeast and the BRCA1 nuclear localization in human cells. Misfolding mutations impair the BRCA1.
The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity.
Degradation of the COPI proteins Ret1 and Sec21 by the AID system.
Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.
Effects of paclitaxel and vinblastine.
Effect of siRNA transfection on colony formation by Renca cells.
Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.
The effect of Bos1 mutations on yeast growth.
2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.
Regulation of Bcl-2 family proteins in the hypothalamus of non-diabetic and diabetic IRS2−/− mice. Regulation of Bcl-2 family proteins in the hypothalamus.
Presentation transcript:

ER-to-Golgi SNARE complex formation with Bos1 p.Arg196del. ER-to-Golgi SNARE complex formation with Bos1 p.Arg196del. (A) SNARE complex formation was measured by the increase of fluorescence anisotropy as previously described (Demircioglu et al., 2014). For assembly, 500 nM OG-labeled Sec22 126-186C131OG were mixed with 2.7 µM Sed5 211-320, Bet1 1-118 and Bos1 151-221 or its mutants. A slower increase in fluorescence anisotropy indicates a reduced capability of Bos1 p.Arg196del to form the ER-to-Golgi SNARE complex. Bos1 p.Gly176Asp shows an even stronger effect. However, complex formation of Bos1 p.Gly176Trp seems to be accelerated. (B) Size exclusion chromatography (SEC) was performed for the individual SNARE domains of Bos1 variants. The Bos1 variants wild-type (wt), p.Gly176Asp and p.Arg196del seemed to be present in a higher oligomeric state than Bos1 p.Gly176Trp. Jörn M. Völker et al. Dis. Model. Mech. 2017;10:1391-1398 © 2017. Published by The Company of Biologists Ltd