Fertility and Sterility

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Presentation transcript:

Fertility and Sterility Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells Ali Salmassi, Ph.D., Andreas G Schmutzler, M.D., Lili Huang, M.D., Jürgen Hedderich, Diplom. Informatics, Walter Jonat, M.D., Liselotte Mettler, M.D. Fertility and Sterility Volume 81, Pages 786-791 (March 2004) DOI: 10.1016/j.fertnstert.2003.09.039

Figure 1 Immunocytochemical demonstration of G-CSF and its receptor (G-CSFR). (a) Granulocyte colony-stimulating factor in the cytoplasma of human follicular GCs. (b) Localization of G-CSFR on the surface of GCs. (c) Granulosa cells without the primary antibody as negative control. (d) Localization of G-CSFR on the surface of monocytes, isolated from blood as positive control. Immunoalkaline phosphatase reaction. Magnification, ×600. Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004. Fertility and Sterility 2004 81, 786-791DOI: (10.1016/j.fertnstert.2003.09.039)

Figure 2 Effect of increasing culture time on G-CSF secretion by granulosa cells (mean G-CSF levels, 95% confidence interval). Results from three experiments (five patients each) performed in triplicate (P<.05). Number of cells, 2 × 104. Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004. Fertility and Sterility 2004 81, 786-791DOI: (10.1016/j.fertnstert.2003.09.039)

Figure 3 In vitro RT-PCR of G-CSF and its receptor (G-CSFR). The lanes show the following. Lane a, The separation of a molecular weight marker; lanes b–d, Granulocyte colony-stimulating factor amplicon in human GCs, in human monocytes, and in human placenta as positive control; lanes e–g, Granulocyte colony-stimulating factor receptor amplicon in human GCs, in human monocytes, and in human placenta as positive control; lane h, Negative control omitting RT; lanes i–k, GAPDH amplicon in human GCs, human monocytes, and placenta. Salmassi. Granulocyte colony-stimulating factor.Fertil Steril 2004. Fertility and Sterility 2004 81, 786-791DOI: (10.1016/j.fertnstert.2003.09.039)