Fertility and Sterility

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Fertility and Sterility Incidence of Fas positivity and deoxyribonucleic acid double-stranded breaks in human ejaculated sperm Carmel M McVicar, Ph.D., Neil McClure, F.R.C.O.G., Kate Williamson, Ph.D., Lauren H Dalzell, B.Sc., Sheena E.M Lewis, Ph.D. Fertility and Sterility Volume 81, Pages 767-774 (March 2004) DOI: 10.1016/j.fertnstert.2003.10.013

FIGURE 1 Fluorescence micrograph displaying Fas positivity on sperm. Fas immunostaining over the head and the midpiece of some abnormally shaped sperm in a teratozoospermic sample. Original magnification, ×1,000. McVicar. Fas and DNA strand breaks in sperm.Fertil Steril 2004. Fertility and Sterility 2004 81, 767-774DOI: (10.1016/j.fertnstert.2003.10.013)

FIGURE 2 Relationship between Fas immunofluorescence in fertile and infertile men's sperm as assessed by flow cytometry. This graph compares the incidence of Fas positivity in fertile (n = 6) and infertile (n = 24) men's sperm. There was a significantly higher incidence of Fas positivity in infertile than in fertile men's sperm (P<.05, t-test). McVicar. Fas and DNA strand breaks in sperm.Fertil Steril 2004. Fertility and Sterility 2004 81, 767-774DOI: (10.1016/j.fertnstert.2003.10.013)

FIGURE 3 Relationship between percent Fas positivity and DNA damage in sperm. There was no relationship between the samples that expressed Fas positivity (columns) and the DSB (OTM >10) (line) (r = 0.14, P=.45, Pearson's correlation). Teratozoospermic, n = 24; normozoospermic, n = 6. McVicar. Fas and DNA strand breaks in sperm.Fertil Steril 2004. Fertility and Sterility 2004 81, 767-774DOI: (10.1016/j.fertnstert.2003.10.013)

FIGURE 4 Relationship between Fas and morphology. There was an inverse relationship between sperm morphology (columns) and the percentage of Fas-positive cells (line) (r = −0.40, P<.05, Pearson's correlation between the Fas positivity of teratozoospermic and normozoospermic samples according to their probit values). Teratozoospermic, n = 24; normozoospermic, n = 6. McVicar. Fas and DNA strand breaks in sperm.Fertil Steril 2004. Fertility and Sterility 2004 81, 767-774DOI: (10.1016/j.fertnstert.2003.10.013)

FIGURE 5 Relationship between Fas and motility. There was an inverse relationship between sperm motility (columns) and the percentage of Fas-positive cells (line) (r = −0.40, P<.05, Pearson's correlation between the Fas positivity of asthenozoospermic and normozoospermic samples according to their probit values). Teratozoospermic, n = 24; normozoospermic, n = 6. McVicar. Fas and DNA strand breaks in sperm.Fertil Steril 2004. Fertility and Sterility 2004 81, 767-774DOI: (10.1016/j.fertnstert.2003.10.013)