Fig. 1. Co-immunostaining of yeast spheroplasts with anti-hASNS and anti-alpha tubulin revealed that yeast asparagine synthetases formed filaments/foci.

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Fig. 1. Co-immunostaining of yeast spheroplasts with anti-hASNS and anti-alpha tubulin revealed that yeast asparagine synthetases formed filaments/foci when grown to saturation and stationary phase. Co-immunostaining of yeast spheroplasts with anti-hASNS and anti-alpha tubulin revealed that yeast asparagine synthetases formed filaments/foci when grown to saturation and stationary phase. (A) Yeast BY4741 cells were grown in YPD at 30°C to saturation (1 day) and stationary phase (5 days), respectively. (B) Yeast asn1Δ and asn2Δ single knockout strains were grown in YPD at 30°C to saturation (1 day). They were all fixed with formaldehyde, lysed their cell walls with zymolase and immunostained with anti-hASNS (red) and anti-alpha tubulin (green), imaged in Z-stack and then compressed into single images using a maximum projection. Chalongrat Noree et al. Biology Open 2018;7:bio038307 © 2018. Published by The Company of Biologists Ltd