Extended micro-organoid culture.

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Volume 18, Issue 6, Pages (June 2016)

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The neuroepithelium of mid-neurulation mammalian embryos displays planar-polarised F-actin profiles. The neuroepithelium of mid-neurulation mammalian embryos.

Figure 2 Comparison of the developing mouse kidney

Volume 18, Issue 6, Pages (June 2016)

The pathophysiological processes linked to kidney disease.

Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Drug‐induced cell death and proliferation inhibition.

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Extended micro-organoid culture. Extended micro-organoid culture. (A) Bright-field images of extended culture of kidney micro-organoids in suspension using hES3-SOX17 mCherry cells on day 7+18, day 7+28 and day 7+41. (B) Confocal immunofluorescence images of different nephron segments on day 7+18, day 7+30 and day 7+40. (C,C′) Confocal immunofluorescence images showing albumin (FITC) uptake at different stages of micro-organoid culture. C′ shows magnified images of the boxed areas in C above. (D) qPCR showing the fold change in gene expression for different nephron segments on day 7+5, day 7+18, day 7+30 and day 7+41 of kidney micro-organoid culture (n=3). Top, podocytes; middle, proximal tubules; bottom, distal tubules and endothelial cells. (E-H) Confocal immunofluorescence images of hES3-SOX17 mCherry micro-organoids after extended culture to day 7+41, illustrating morphological changes. Dysplastic organoids are stained for different segments of nephron and stoma (E), show proliferation within an expanding stromal population (F), and evidence of apoptotic cells (CASP3+) (G) and fibrotic (α-SMA) lesions (H). Scale bars: 100 µm (A); 50 µm (B,E-H); 20 µm (C); 5 µm (C′). Santhosh V. Kumar et al. Development 2019;146:dev172361 © 2019. Published by The Company of Biologists Ltd