Overexpression of Nedd4 is able to rescue miR-1-mediated actin fiber disarray in larval hearts. Overexpression of Nedd4 is able to rescue miR-1-mediated.

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Overexpression of Nedd4 is able to rescue miR-1-mediated actin fiber disarray in larval hearts. Overexpression of Nedd4 is able to rescue miR-1-mediated actin fiber disarray in larval hearts. (A) Wild-type larval (L3) heart stained with muscle-associated actin filaments. (B) Overexpression of miR-1 in the fly heart using the Hand>UAS-miR-1 expression construct results in actin filament disorganization and collapse. (C) Concurrent expression of Nedd4 with miR-1 (Hand>UAS-Myc-Nedd4+UAS-miR-1) significantly normalizes actin fiber organization. (D) Concurrent expression of a ubiquitin ligase-deficient mutant of Nedd4 with miR-1 [Hand>UAS-Myc-Nedd4(C/A)+UAS-miR-1] improves actin fiber organization. (E,F) Overexpression of Nedd4 (Hand>UAS-Myc-Nedd4) or mutant Nedd4 [Hand>UAS-Myc-Nedd4(C/A)] in larval hearts has minimal effects on actin fiber organization. All larvae were raised at 29°C. n=6 for each genotype. Jun-yi Zhu et al. Development 2017;144:866-875 © 2017. Published by The Company of Biologists Ltd