Volume 8, Issue 12, Pages (December 2001)

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Volume 8, Issue 12, Pages 1239-1252 (December 2001) Elucidation of the function of two glycosyltransferase genes (lanGT1 and lanGT4) involved in landomycin biosynthesis and generation of new oligosaccharide antibiotics Axel Trefzer, Carsten Fischer, Sigrid Stockert, Lucy Westrich, Eva Künzel, Ulrich Girreser, Jürgen Rohr, Andreas Bechthold Chemistry & Biology Volume 8, Issue 12, Pages 1239-1252 (December 2001) DOI: 10.1016/S1074-5521(01)00091-6

Fig. 1 Structures of landomycin A produced by S. cyanogenus S136 and urdamycin derivatives produced by S. fradiae and mutants S. fradiae A-x and S. fradiae A-0. For the urdamycins only A- and B-type aglycones are shown. These are produced as a complex with C- and D-type urdamycins without changes in the sugar moieties [21]. Chemistry & Biology 2001 8, 1239-1252DOI: (10.1016/S1074-5521(01)00091-6)

Fig. 2 Dendrogram derived from the comparison of urdamycin and landomycin glycosyltransferase sequences (accession numbers: UrdGT1a AAF00214, UrdGT1b AAF00215, UrdGT1c AAF00217, UrdGT2 AAF00209, LanGT1 AAD13555, LanGT2 AAD13553, LanGT3 AAD13559, LanGT4 AAD13562). The dendrogram was drawn based on an alignment built with DNASIS using the resettings of the program. Genetic similarity is given at points of branching. Chemistry & Biology 2001 8, 1239-1252DOI: (10.1016/S1074-5521(01)00091-6)

Fig. 3 A: Organization of the urdamycin biosynthetic gene cluster from S. fradiae Tü2717. Genes and their direction of transcription are indicated by arrows; active glycosyltransferase genes are shown in red, polyketide synthase genes in blue; the blue bar shows the localization of the fragment used as probe for hybridization; only relevant restriction sites are shown. B: Genotypes of glycosyltransferase gene mutant strains used in this study; inactive glycosyltransferase genes are shown in dark gray. C: Southern hybridization confirming the deletion in S. fradiae A-0; lane M: DIG-labelled molecular size marker with the following fragments shown: 1953 bp, 2799 bp, 3639 bp, 4899 bp, 6106 bp; lane 1: chromosomal DNA isolated from S. fradiae BF-1-1, digested with PstI; lane 2: chromosomal DNA isolated from S. fradiae A-0, digested with PstI (for details see text). Chemistry & Biology 2001 8, 1239-1252DOI: (10.1016/S1074-5521(01)00091-6)

Fig. 4 Generation of a gene cassette containing lanGT1 and lanGT4 using the pMUN vector system. Chemistry & Biology 2001 8, 1239-1252DOI: (10.1016/S1074-5521(01)00091-6)

Fig. 5 Compounds produced by expression of glycosyltransferase genes in mutants S. fradiae A-x and S. fradiae A-0. Chemistry & Biology 2001 8, 1239-1252DOI: (10.1016/S1074-5521(01)00091-6)