Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions.

Slides:

Advertisements

Similar presentations

A novel wheat gliadin as a cause of exercise-induced anaphylaxis

Advertisements

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.) Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Napins, 2S albumins, are major allergens in oilseed rape and turnip rape Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,

Contamination of probiotic preparations with milk allergens can cause anaphylaxis in children with cow's milk allergy Tzu-Tai Tiger Lee, PhD, Martine.

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Lupine allergy: Not simply cross-reactivity with peanut or soy

Wheat α-amylase inhibitor: A second route of allergic sensitization

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Gloria García-Casado, PhD, Jesús F

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Hypersensitivity reactions due to nitrile gloves

Christiane Kruse Fæste, PhD, Ellen Namork, PhD, Helene Lindvik, MMSc

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Rhinoconjunctival sensitization to hydrolyzed wheat protein in facial soap can induce wheat-dependent exercise-induced anaphylaxis Yuma Fukutomi, MD,

Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Is 9 more than 2 also in allergic airway inflammation?

Allergy to eggplant (Solanum melongena)

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

Leticia Vila, MD, PhD, Borja Bartolome, PhD, Ana Moreno, MD

Identification of IgE-binding components of citrus red mite in sera of patients with citrus red mite–induced asthma Hee-Yeon Kim, MDa, Hae-Sim Park,

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Allergy to eggs from duck and goose without sensitization to hen egg proteins Belén Añíbarro, MD, F.Javier Seoane, MD, Concepción Vila, MD, Manuel Lombardero,

Anaphylactic reaction to (mixed) fruit juice containing dragon fruit

Morten J. Christensen, MD, Per Stahl Skov, MD, DMSci, Lars K

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian.

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen Masako Toda, PhD, Gerald Reese, PhD, Gabriele.

Influence of refining steps on trace allergenic protein content in sunflower oil Nedjma Zitouni, PhD, Younes Errahali, MS, Maurice Metche, PhD, Gisèle.

Respiratory sensitization of a worker to the warehouse beetle Trogoderma variabile: An index case report Jonathan A. Bernstein, MD, Marjorie S. Morgan,

Component-resolved diagnosis of kiwifruit allergy with purified natural and recombinant kiwifruit allergens Merima Bublin, PhD, Marina Pfister, MD, Christian.

The brown marmorated stinkbug as a new aeroallergen

Tolerance to wheat in whole-grain cereal biscuit in wheat-allergic children Paul J. Turner, FRACP, PhD, Melanie Wong, FRACP, PhD, Nirupama Varese, MSc,

Clinical characteristics of soybean allergy in Europe: A double-blind, placebo-controlled food challenge study Barbara K. Ballmer-Weber, MD, Thomas Holzhauser,

Kerstin Bauermeister, MSc, Barbara K

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1 Christiane Hilger, PhD,

Melanie Albrecht, MSc, Yvonne Kühne, MSc, Barbara K

Systemic allergic reaction to coconut (Cocos nucifera) in 2 subjects with hypersensitivity to tree nut and demonstration of cross-reactivity to legumin-like.

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7

Anaphylaxis caused by hidden soybean allergens in pillows

Casein-related anaphylaxis after use of an Everlast kickboxing glove

Component-resolved in vitro diagnosis of hazelnut allergy in Europe

Immune responses to mosquito saliva in 14 individuals with acute systemic allergic reactions to mosquito bites Zhikang Peng, MD, Andrew N. Beckett, BSc,

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Soybean allergy in patients allergic to birch pollen

Anaphylaxis to apple and orange seed

Anna Nowak-Wegrzyn, MD, Gail G

Beer anaphylaxis Journal of Allergy and Clinical Immunology

Isolation and biochemical characterization of a thaumatin-like kiwi allergen Marija Gavrović-Jankulović, PhDa, Tanja Ćirković, MSa, Olga Vučković, MDb,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

α-Purothionin, a new wheat allergen associated with severe allergy

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Recombinant allergen-based IgE testing to distinguish bee and wasp allergy Irene Mittermann, PhD, Mihaela Zidarn, MD, Mira Silar, PhD, Zora Markovic-Housley,

Cat IgA, representative of new carbohydrate cross-reactive allergens

Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens Juan A. Asturias, PhD, Ignacio Ibarrola, PhD, Angel Ferrer,

Allergy to human seminal fluid: Cross-reactivity with dog dander

Cloning, sequencing, and recombinant production of Sin a 2, an allergenic 11S globulin from yellow mustard seeds Oscar Palomares, PhD, Andrea Vereda,

Pınar Uysal, MD, Esben Eller, MSc, PhD, Charlotte G

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries Merima Bublin, MSc,

The effects of gastric digestion on codfish allergenicity

Natural history of cow’s milk allergy

Presentation transcript:

Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy Thomas Holzhauser, PhD, Olga Wackermann, MSc, Barbara K. Ballmer-Weber, MD, Carsten Bindslev-Jensen, MD, Joseph Scibilia, MD, Lorenza Perono-Garoffo, PhD, Shigeru Utsumi, PhD, Lars K. Poulsen, PhD, Stefan Vieths, PhD Journal of Allergy and Clinical Immunology Volume 123, Issue 2, Pages 452-458.e4 (February 2009) DOI: 10.1016/j.jaci.2008.09.034 Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 IgE-immunoblot analysis of a soybean extract. All lanes except for 28∗ and P were detected with peroxidase mediated chemiluminescence (lane C: buffer control; lane N: nonatopic control serum pool [n = 3]; lanes 1, 3, 14, 18, 21, 30, and 28: sera from subjects with soybean allergy summarized in Table I; lane 28∗: patient 28 detected with phosphatase and precipitating dye; lane P: silver-stained total soybean protein). Journal of Allergy and Clinical Immunology 2009 123, 452-458.e4DOI: (10.1016/j.jaci.2008.09.034) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Purity testing of the individual Gly m 5 (β-conglycinin) subunits β (Gly m 5.03), α (Gly m 5.01), and α′ (Gly m 5.02) from natural soybean source in SDS-PAGE and silver staining (lanes β, α, and α′), and verification of the IgE reactivity of all Gly m 5 subunits by immunoblot analysis with selected soy allergic sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 5, 14, 18, 19, and 20: subjects with soybean allergy). Journal of Allergy and Clinical Immunology 2009 123, 452-458.e4DOI: (10.1016/j.jaci.2008.09.034) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

A, Individual subunits of Gly m 6 by total protein staining of Western blots with Ponceau S (lanes: G1, Gly m 6.01, G2, Gly m 6.02; G3, Gly m 6.03; G4, Gly m 6.04; G5, Gly m 6.05) and verification of IgE-reactivity of all Gly m 6 subunits (double lane) with patient 28 (N: nonatopic serum pool). B, IgE immunoblot analysis of equal amounts (wt/wt) of the Gly m 6 subunits with selected patients' sera (lane C: buffer control; N: nonatopic control serum pool [n = 3]; lanes 1, 12, 14, 19, and 21: subjects with soybean allergy). The arrows indicate the position of the 5 proglycinin subunits according to molecular weight. Journal of Allergy and Clinical Immunology 2009 123, 452-458.e4DOI: (10.1016/j.jaci.2008.09.034) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions