EcR is not required for DHR3 and βFtz-f1 expression.

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EcR is not required for DHR3 and βFtz-f1 expression. EcR is not required for DHR3 and βFtz-f1 expression. (A-L) EcR-RNAi flip-out clones marked by GFP (A,D,G,J, arrowheads in the corresponding pictures) were stained for EcR (C,F,I,L) and DHR3 (B,E) or βFtz-f1 (H,K) in ring glands of either L2 (A-C,G-I) or L3 (D-F,J-L) larvae. Nuclear labelling (A,D,G,J). (M-P) βFtz-f1 expression (N,P) in homozygous DHR3G60S mutant clones in ring glands analysed at late L3 (M,N) or late L2 (O,P) stages. Clones are labelled by the lack of GFP (dashed lines in M,O and arrowheads in N,P). Scale bars: 20 µm. (Q) βFtz-f1 expression in homozygous DHR3G60S mutant clones. The percentages of the various phenotypes are represented as proportional bars corresponding to either a decrease in (red) or no effect (green) on βFtz-f1 expression. βFtz-f1 levels are indicated at the top. Number of ring glands analysed that exhibited DHR3G60S mutant clones are indicated for each developmental stage (n). In all pictures of clones, the timing of the heat shock (stageX) and of the dissection (stageY) is indicated as HSstageX≫stageY; developmental stages: embryogenesis (E), L1 stage (L1), L2 stage past the 20E peak (L2), late L2 stage (L2l), L2/L3 transition (L2/L3), early (L3e) or late (L3l) L3 stages. Jean-Philippe Parvy et al. Development 2014;141:3955-3965 © 2014. Published by The Company of Biologists Ltd