Induction of monocyte chemotactic protein-1 in peritoneal mesothelial and endometrial cells by oxidized low-density lipoprotein and peritoneal fluid from.

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Induction of monocyte chemotactic protein-1 in peritoneal mesothelial and endometrial cells by oxidized low-density lipoprotein and peritoneal fluid from women with endometriosis Rong Rong, Ph.D., M.D., Sumathi Ramachandran, Ph.D., Nalini Santanam, Ph.D., Ana A Murphy, M.D., Sampath Parthasarathy, Ph.D. Fertility and Sterility Volume 78, Issue 4, Pages 843-848 (October 2002) DOI: 10.1016/S0015-0282(02)03333-2

FIGURE 1 MCP-1 levels in the medium, secreted by mesothelial cells, EM42 cells, or stromal cells after incubation with native LDL or oxidized LDL. Cells were incubated with 25 μg/mL LDL, moLDL (minimally oxidized LDL), or oxLDL (Oxidized LDL) for 6 to 8 hours. The MCP-1 levels in the medium were measured by ELISA assay and expressed in terms of total cellular protein. The mean ± SE of five experiments is shown in the figure. Statistical comparisons were made with reference to control. ∗P<.05. Rong. Oxidative stress and MCP-1 in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 843-848DOI: (10.1016/S0015-0282(02)03333-2)

FIGURE 2 MCP-1 levels in the medium, secreted by endometrial cells after incubation with peritoneal fluid (PF). For 2 to 3 hours 10% peritoneal fluid was cultured with or without EM42 cells or stromal cells. The MCP-1 levels in the medium were measured by ELISA assay and expressed in terms of total cellular protein. The mean ± SE of three experiments is shown in the figure. Statistical comparisons were made with reference to the corresponding groups, which were incubated with peritoneal fluid from normal women. ∗∗P<.01. Rong. Oxidative stress and MCP-1 in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 843-848DOI: (10.1016/S0015-0282(02)03333-2)

FIGURE 3 MCP-1 mRNA levels of mesothelial cells, EM42 cells, or stromal cells after incubation with native LDL or oxidized LDL. Cells were incubated with 25 μg/mL LDL, moLDL (minimally oxidized LDL), or oxLDL (oxidized LDL) for 6 to 8 hours, then RNA was harvested and analyzed. (A) A representative agarose gel. (B) The graphic representation of the average results from three separate experiments after correction using GAPDH. Data are mean ± SE of three separate experiments. ∗P<.05. Rong. Oxidative stress and MCP-1 in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 843-848DOI: (10.1016/S0015-0282(02)03333-2)