Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

Slides:

Advertisements

Similar presentations

Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao

Advertisements

Differentiation of AZD4785 from MAPK pathway inhibitors in vitro

Ahyeon Son, Jong-Eun Park, V. Narry Kim Cell Reports

Fig. 6. HERV-K activation by TDP-43 and identification of binding sites on LTR. (A and B) Stem cell–derived neurons were transfected with either pcDNA.

CHK1 downregulation upon ERG overexpression.

TSA dose-dependent increase in transcription.

mRNA level as % relative to

Bernard et al, Supplementary Figure S1

Fold Change of hsa-miR-3687 (T/N)(log2)

Volume 9, Issue 5, Pages (November 2017)

Differential expression levels of archaella operon genes and respiratory chain genes in Δsaci_pp2a and Δsaci_ptp. Differential expression levels of archaella.

Effect of small TbTim knockdown on cell growth.

Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc

Change in mRNA expression of Glasso network genes in differentiated PC12 cells following Caprin2 knockdown or Caprin2 overexpression. Change in mRNA expression.

qRT-PCR validation of differential expression.

Volume 13, Issue 10, Pages (December 2015)

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

Fig. 2. Effect of endurance training on gene expression, and protein content and activity in heart muscle. Effect of endurance training on gene expression,

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 1. Muscle-specific PITX1 over-expression in the Pitx1 transgenic mice.(A) Detection of Pitx1 mRNA expression in muscles of the Pitx1 transgenic mice.

Fig. 2. p85β regulates cell adhesion

Fig. 4. Expression of miR-2 family mediates tissue growth of Hippo pathway.(A) Photomicrographs of adult wings of the indicated genotype. Expression of.

Fig. 2. p53 mediates the effects of Yorkie

Fig. 1. Loss of PC following INT depletion

Effect of YopM on the transcription of pro-inflammatory cytokines in HL60-derived macrophages after stimulation with LPS. HL60 cells differentiated into.

Fig. 1. Coilin interaction with the telomerase complex protein dyskerin is mediated by hTR. Coilin interaction with the telomerase complex protein dyskerin.

Fig. 2. DDR1 over-expression enhances collagen fibril reorganization

Fig. 7. SCF and IGF-1 accelerate fetal spleen erythropoiesis in vivo.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fig. 3. Characterization of unclassified cells (UCs).

Fig. 7. ML mediated suppression of SAHA-induced up-regulation of tRNA as a potential mechanism that contributes to the enhanced anti-cancer actions.

The TER94-p47 complex isinvolved in Notch signaling regulation

Fig. 6. Coilin mutants display differential hTR processing activity but lack specificity toward the 5′ or 3′ regions.(A) Illustration of human telomerase.

CTG repeats do not affect the levels of TBPH transcripts nor TBPH alternative splicing. CTG repeats do not affect the levels of TBPH transcripts nor TBPH.

Fig. 4. Coilin phosphomutant displays differential RNA binding and degradation activities.Purified proteins were analyzed for RNase activity with total.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Fig. 2. STK35 RNAs are expressed in distinct yet overlapping cell populations in adult mouse testis. STK35 RNAs are expressed in distinct yet overlapping.

Fig. 6. STK35 KO mice show ovary defects.

P3/QBP1 co-treatment suppressed expanded-CAG-RNA-induced RNA toxicity and expanded-polyQ-protein-induced protein toxicity in vivo. P3/QBP1 co-treatment.

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Effect of the plastid translation inhibitor Spec on LR development

Loss or increase of Tj causes a reduction in Slbo expression.

Fig. 1. Loss of PC following INT depletion

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effect of Dipy on the expression of chondrogenic markers.

Nrf2−/− mice had higher TGF-β1 transcription and FN expression.

Depletion of INTS11 produces a similar phenotype to that observed upon depletion of INTS4. Depletion of INTS11 produces a similar phenotype to that observed.

Fig. 4. Impaired induction of TH-response genes in trβ crispants

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

Fig. 6 Functional characterization of RdRp activity of the recMeV mutants with modified N proteins. Functional characterization of RdRp activity of the.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro. Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro.

Fig. 5. Coilin mutants display differential U2 processing activity with specificity toward the CU region.Purified proteins were incubated with in vitro.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Proliferative and morphological defects following TbCALM and TbEpsinR depletion. Proliferative and morphological defects following TbCALM and TbEpsinR.

BCL-3 regulates expression of stemness-associated Wnt targets.

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Figure 2 LMNB1 mRNA expression

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

Fig. 1 Dcr-2 is required for Toll signaling in Drosophila S2 cells.

Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte differentiation. Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte.

Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

SUPT4H1 Depletion Leads to a Global Reduction in RNA

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

Presentation transcript:

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin was analyzed by qRT-PCR using primers specific to U2 snRNA, telomerase RNA and rRNA. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin was analyzed by qRT-PCR using primers specific to U2 snRNA, telomerase RNA and rRNA. (A) Histogram of relative 47/45S precursor rRNA to 5.8S rRNA following 48-hour control or coilin knockdown in HeLa cells resulting in 87% reduction of coilin mRNA. (B) Histogram of the fold change of specific RNA transcripts following 48-hour coilin knockdown in WI-38 cells resulting in an average 96% reduction of coilin mRNA; *P<0.05 relative to control knockdown; #P<0.05 between data pairs. (C) Histogram of coilin expression, relative to GAPDH, in HeLa and WI-38 cells treated with control siRNA for 48 hours; *P<0.05 relative to HeLa. Error bars represent 1 s.d. of 3 experimental repeats (2 for WI-38 hTR) and 3 technical repeats within each experiment. All message levels were normalized to GAPDH prior to comparison with either control knockdown or between cell lines. Hanna J. Broome et al. Biology Open 2013;2:407-415 © 2013. Published by The Company of Biologists Ltd